Expression of TRAIL-splice variants in gastric carcinomas: identification of TRAIL-γ as a prognostic markerReportar como inadecuado




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BMC Cancer

, 13:384

Translational oncology

Abstract

BackgroundTNF-related apoptosis inducing ligand TRAIL belongs to the TNF-superfamily that induces apoptotic cell death in a wide range of neoplastic cells in vivo as well as in vitro. We identified two alternative TRAIL-splice variants, i.e. TRAIL-β and TRAIL-γ that are characterized by the loss of their proapoptotic properties. Herein, we investigated the expression and the prognostic values of the TRAIL-splice variants in gastric carcinomas.

MethodsReal time PCR for amplification of the TRAIL-splice variants was performed in tumour tissue specimens and corresponding normal tissues of 41 consecutive patients with gastric carcinoma. Differences on mRNA-expression levels of the TRAIL-isoforms were compared to histo-pathological variables and correlated with survival data.

ResultsAll three TRAIL-splice variants could be detected in both non-malignant and malignant tissues, irrespective of their histological staging, grading or tumour types. However, TRAIL-β exhibited a higher expression in normal gastric tissue. The proapoptotic TRAIL-α expression was increased in gastric carcinomas when compared to TRAIL-β and TRAIL-γ. In addition, overexpression of TRAIL-γ was associated with a significant higher survival rate.

ConclusionsThis is the first study that investigated the expression of TRAIL-splice variants in gastric carcinoma tissue samples. Thus, we provide first data that indicate a prognostic value for TRAIL-γ overexpression in this tumour entity.

KeywordsTRAIL Alternative splicing Apoptosis Gastric carcinoma Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-13-384 contains supplementary material, which is available to authorized users.

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Fuente: https://link.springer.com/







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