Immunofluorescence labeling of cell surface antigens in DictyosteliumReportar como inadecuado




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BMC Research Notes

, 6:317

First Online: 12 August 2013Received: 08 May 2013Accepted: 09 August 2013DOI: 10.1186-1756-0500-6-317

Cite this article as: Vernay, A. & Cosson, P. BMC Res Notes 2013 6: 317. doi:10.1186-1756-0500-6-317

Abstract

BackgroundImmunolocalization of cellular antigens typically requires fixation and permeabilization of cells, prior to incubation with antibodies.

FindingsAssessing a test protein abundantly present at the cell surface of Dictyostelium cells, we show that in fixed cells, permeabilization extracts almost completely this cell surface antigen. The extent of this artifact is variable depending on the procedure used for labeling and permeabilization, as well as on the antigen considered.

ConclusionsAn optimized protocol for labeling both surface and intracellular antigens without significant loss of labeling is proposed.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-6-317 contains supplementary material, which is available to authorized users.

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Autor: Alexandre Vernay - Pierre Cosson

Fuente: https://link.springer.com/



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