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BMC Research Notes

, 6:386

First Online: 27 September 2013Received: 04 September 2013Accepted: 25 September 2013DOI: 10.1186-1756-0500-6-386

Cite this article as: Uzbekov, R. & Roingeard, P. BMC Res Notes 2013 6: 386. doi:10.1186-1756-0500-6-386


BackgroundRecent studies have suggested that nuclear lipid droplets LDs are organized into domains similar to those of cytoplasmic LDs. As cytoplasmic LDs are formed at the endoplasmic reticulum ER membrane, which is structurally continuous with the nuclear envelope, it could be suggested however that nuclear LDs are cytoplamic LDs trapped within an invagination of the nuclear envelope. The resolution of fluorescence confocal microscopy is not sufficiently high to exclude this hypothesis.

FindingsWe therefore addressed this question by electron microscopy EM of serial sections. In human liver tissue, we observed some cytoplamic LDs partly surrounded by the nuclear compartment, but we were also able to identify LDs residing in the nuclear compartment that were not connected to the nuclear envelope.

ConclusionThese findings indicate that nuclear LDs constitute specific subdomains of the nuclear compartment probably involved in nuclear lipid homeostasis.

KeywordsLipid droplet Nucleus Electron microscopy EM tomography AbbreviationsEMElectron microscopy

EREndoplasmic reticulum

HCVHepatitis C virus

LDLipid droplet.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-6-386 contains supplementary material, which is available to authorized users.

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Autor: Rustem Uzbekov - Philippe Roingeard


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