CD26 Expression on T-Anaplastic Large Cell Lymphoma ALCL Line Karpas 299 is associated with increased expression of Versican and MT1-MMP and enhanced adhesionReportar como inadecuado




CD26 Expression on T-Anaplastic Large Cell Lymphoma ALCL Line Karpas 299 is associated with increased expression of Versican and MT1-MMP and enhanced adhesion - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Cancer

, 13:517

Cell and molecular biology

Abstract

BackgroundCD26-dipeptidyl peptidase IV DPPIV is a multifunctional membrane protein with a key role in T-cell biology and also serves as a marker of aggressive cancers, including T-cell malignancies.

MethodsVersican expression was measured by real-time RT-PCR and Western blots. Gene silencing of versican in parental Karpas 299 cells was performed using transduction-ready viral particles. The effect of versican depletion on surface expression of MT1-MMP was monitored by flow cytometry and surface biotinylation. CD44 secretion-cleavage and ERK 1-2 activation was followed by Western blotting. Collagenase I activity was measured by a live cell assay and in vesicles using a liquid-phase assay. Adhesion to collagen I was quantified by an MTS assay.

ResultsVersican expression was down-regulated in CD26-depleted Karpas 299 cells compared to the parental T-ALCL Karpas 299 cells. Knock down of versican in the parental Karpas 299 cells led to decreased MT1-MMP surface expression as well as decreased CD44 expression and secretion of the cleaved form of CD44. Parental Karpas 299 cells also exhibited higher collagenase I activity and greater adhesion to collagenase I than CD26-knockdown or versican-knockdown cells. ERK activation was also highest in parental Karpas 299 cells compared to CD26-knockdown or versican-knockdown clones.

ConclusionsOur data indicate that CD26 has a key role in cell adhesion and invasion, and potentially in tumorigenesis of T-cell lines, through its association with molecules and signal transduction pathways integral to these processes.

KeywordsCD26 T-cell malignancies Adhesion MT1-MMP Cell signaling Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-13-517 contains supplementary material, which is available to authorized users.

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Autor: Pamela A Havre - Long H Dang - Kei Ohnuma - Satoshi Iwata - Chikao Morimoto - Nam H Dang

Fuente: https://link.springer.com/



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