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Journal of Translational Medicine

, 7:100

First Online: 26 November 2009Received: 19 June 2009Accepted: 26 November 2009DOI: 10.1186-1479-5876-7-100

Cite this article as: Lehmann, L.E., Book, M., Hartmann, W. et al. J Transl Med 2009 7: 100. doi:10.1186-1479-5876-7-100

Abstract

BackgroundMacrophage migration inhibitory factor MIF plays an important regulatory role in sepsis. In the promoter region a C-G single nucleotide polymorphism SNP at position -173 rs755622 and a CATT5-8 microsatellite at position -794 are related to modified promoter activity. The purpose of the study was to analyze their association with the incidence and outcome of severe sepsis.

MethodsGenotype distributions and allele frequencies in 169 patients with severe sepsis, 94 healthy blood donors and 183 postoperative patients without signs of infection or inflammation were analyzed by real time PCR and Sequence analysis. All included individuals were Caucasians.

ResultsGenotype distribution and allele frequencies of severe sepsis patients were comparable to both control groups. However, the genotype and allele frequencies of both polymorphisms were associated significantly with the outcome of severe sepsis. The highest risk of dying from severe sepsis was detectable in patients carrying a haplotype with the alleles -173 C and CATT7 p = 0.0005, fisher exact test, RR = 1,806, CI: 1.337 to 2.439.

ConclusionThe haplotype with the combination of the -173 C allele and the -794 CATT7 allele may not serve as a marker for susceptibility to sepsis, but may help identify septic patients at risk of dying.

List of AbbreviationsAP-1Activator protein-1

CIConfidence interval

ICUIntensive care unit

IL-6Interleukin-6

MIFMacrophage migration inhibitory factor

n.a.not applicable

PCRPolymerase chain reaction

PCTProcalcitonin

RRRelative risk

SNPSingle nucleotide polymorphism

SOFASequential Organ Failure Assessment

TLR4Toll like receptor 4.

Lutz E Lehmann, Malte Book contributed equally to this work.

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Autor: Lutz E Lehmann - Malte Book - Wolfgang Hartmann - Stefan U Weber - Jens-Christian Schewe - Sven Klaschik - Andreas Hoeft

Fuente: https://link.springer.com/



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