Let-7 microRNAs are developmentally regulated in circulating human erythroid cellsReportar como inadecuado




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Journal of Translational Medicine

, 7:98

First Online: 25 November 2009Received: 12 November 2009Accepted: 25 November 2009DOI: 10.1186-1479-5876-7-98

Cite this article as: Noh, SJ., Miller, S.H., Lee, Y.T. et al. J Transl Med 2009 7: 98. doi:10.1186-1479-5876-7-98

Abstract

BackgroundMicroRNAs are ~22nt-long small non-coding RNAs that negatively regulate protein expression through mRNA degradation or translational repression in eukaryotic cells. Based upon their importance in regulating development and terminal differentiation in model systems, erythrocyte microRNA profiles were examined at birth and in adults to determine if changes in their abundance coincide with the developmental phenomenon of hemoglobin switching.

MethodsExpression profiling of microRNA was performed using total RNA from four adult peripheral blood samples compared to four cord blood samples after depletion of plasma, platelets, and nucleated cells. Labeled RNAs were hybridized to custom spotted arrays containing 474 human microRNA species miRBase release 9.1. Total RNA from Epstein-Barr virus EBV-transformed lymphoblastoid cell lines provided a hybridization reference for all samples to generate microRNA abundance profile for each sample.

ResultsAmong 206 detected miRNAs, 79% of the microRNAs were present at equivalent levels in both cord and adult cells. By comparison, 37 microRNAs were up-regulated and 4 microRNAs were down-regulated in adult erythroid cells fold change > 2; p < 0.01. Among the up-regulated subset, the let-7 miRNA family consistently demonstrated increased abundance in the adult samples by array-based analyses that were confirmed by quantitative PCR 4.5 to 18.4 fold increases in 6 of 8 let-7 miRNA. Profiling studies of messenger RNA mRNA in these cells additionally demonstrated down-regulation of ten let-7 target genes in the adult cells.

ConclusionThese data suggest that a consistent pattern of up-regulation among let-7 miRNA in circulating erythroid cells occurs in association with hemoglobin switching during the fetal-to-adult developmental transition in humans.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-7-98 contains supplementary material, which is available to authorized users.

Seung-Jae Noh, Samuel H Miller contributed equally to this work.

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Autor: Seung-Jae Noh - Samuel H Miller - Y Terry Lee - Sung-Ho Goh - Francesco M Marincola - David F Stroncek - Christopher Re

Fuente: https://link.springer.com/







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