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Journal of Translational Medicine

, 7:91

First Online: 29 October 2009Received: 11 May 2009Accepted: 29 October 2009DOI: 10.1186-1479-5876-7-91

Cite this article as: Viscor, G., Javierre, C., Pagès, T. et al. J Transl Med 2009 7: 91. doi:10.1186-1479-5876-7-91

Abstract

BackgroundOur goal was to determine whether short-term intermittent hypoxia exposure, at a level well tolerated by healthy humans and previously shown by our group to increase EPO and erythropoiesis, could mobilize hematopoietic stem cells HSC and increase their presence in peripheral circulation.

MethodsFour healthy male subjects were subjected to three different protocols: one with only a hypoxic stimulus OH, another with a hypoxic stimulus plus muscle electrostimulation HME and the third with only muscle electrostimulation OME. Intermittent hypobaric hypoxia exposure consisted of only three sessions of three hours at barometric pressure 540 hPa equivalent to an altitude of 5000 m for three consecutive days, whereas muscular electrostimulation was performed in two separate periods of 25 min in each session. Blood samples were obtained from an antecubital vein on three consecutive days immediately before the experiment and 24 h, 48 h, 4 days and 7 days after the last day of hypoxic exposure.

ResultsThere was a clear increase in the number of circulating CD34+ cells after combined hypobaric hypoxia and muscular electrostimulation. This response was not observed after the isolated application of the same stimuli.

ConclusionOur results open a new application field for hypobaric systems as a way to increase efficiency in peripheral HSC collection.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-7-91 contains supplementary material, which is available to authorized users.

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Autor: Ginés Viscor - Casimiro Javierre - Teresa Pagès - Josep-Lluis Ventura - Antoni Ricart - Gregorio Martin-Henao - Carmen Az

Fuente: https://link.springer.com/







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