The Type VI Secretion System Encoded in SPI-6 Plays a Role in Gastrointestinal Colonization and Systemic Spread of Salmonella enterica serovar Typhimurium in the ChickenReportar como inadecuado




The Type VI Secretion System Encoded in SPI-6 Plays a Role in Gastrointestinal Colonization and Systemic Spread of Salmonella enterica serovar Typhimurium in the Chicken - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Resumen

The role of the Salmonella Pathogenicity Islands SPIs in pathogenesis of Salmonella enterica Typhimurium infection in thechicken is poorly studied, while many studies have been completed in murine models. The Type VI Secretion System T6SSis a recently described protein secretion system in Gram-negative bacteria. The genus Salmonella contains fivephylogenetically distinct T6SS encoded in differentially distributed genomic islands. S. Typhimurium harbors a T6SSencoded in SPI-6 T6SSSPI-6, which contributes to the ability of Salmonella to colonize mice. On the other hand, serotypeGallinarum harbors a T6SS encoded in SPI-19 T6SSSPI-19 that is required for colonization of chicks. In this work, weinvestigated the role of T6SSSPI-6 in infection of chicks by S. Typhimurium. Oral infection of White Leghorn chicks showedthat a DT6SSSPI-6 mutant had reduced colonization of the gut and internal organs, compared with the wild-type strain.Transfer of the intact T6SSSPI-6 gene cluster into the T6SS mutant restored bacterial colonization. In addition, our resultsshowed that transfer of T6SSSPI-19 from S. Gallinarum to the DT6SSSPI-6 mutant of S. Typhimurium not only complementedthe colonization defect but also resulted in a transient increase in the colonization of the cecum and ileum of chicks at days1 and 3 post-infection. Our data indicates that T6SSSPI-6 contributes to chicken colonization and suggests that both T6SSSPI-6and T6SSSPI-19 perform similar functions in vivo despite belonging to different phylogenetic families.Nota general

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