Auxiliary tools in tuberculosis. the hemolysis in pleural fluids underestimate the values of adenosine deaminase activity determined by the method of giusti Reportar como inadecuado




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C. RIVAS ;Revista Argentina de Microbiología 2008, 40 2

Autor: C. COITINHO

Fuente: http://www.redalyc.org/


Introducción



Revista Argentina de Microbiología ISSN: 0325-7541 ram@aam.org.ar Asociación Argentina de Microbiología Argentina COITINHO, C.; RIVAS, C. Auxiliary tools in tuberculosis.
The hemolysis in pleural fluids underestimate the values of adenosine deaminase activity determined by the method of Giusti Revista Argentina de Microbiología, vol.
40, núm.
2, 2008, pp.
101-105 Asociación Argentina de Microbiología Buenos Aires, Argentina Available in: http:--www.redalyc.org-articulo.oa?id=213016787006 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Hemolysis underestimate the adenosine deaminase in pleural fluids ARTÍCULO ORIGINAL 101 ISSN 0325-7541 Revista Argentina de Microbiología (2008) 40: 101-105 Auxiliary tools in tuberculosis.
The hemolysis in pleural fluids underestimate the values of adenosine deaminase activity determined by the method of Giusti C.
COITINHO*, C.
RIVAS Comisión Honoraria para la Lucha Antituberculosa y Enfermedades Prevalentes.
Centro de Referencia Nacional para Micobacterias.
Dpto.
de Laboratorio.
Avda.
18 de Julio 2175 6p.
Montevideo, Uruguay. *Correspondencia: E-mail: ccoitinho@yahoo.com. ABSTRACT The increase of adenosine deaminase (ADA) activity in pleural fluids (PF) is considered a useful tool in the diagnosis of pleural tuberculosis.
It is known that numerous photometric methods are interfered by the hemolysis, as a result, hemolyzed specimens –or with blood– received in the laboratory are frequently rejected.
In order to establish if the values of ADA were affected by the hemolysis or blood, ADA was determined in individual and pooled PF samples with the aggregate of erythrocyte lysate (H) or hemolyzed whole blood (HWB) from 312 mg-l to 12 g-l (final concentrations of hemoglobin in the sam...





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