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BMC Research Notes

, 7:160

First Online: 19 March 2014Received: 03 June 2013Accepted: 13 March 2014DOI: 10.1186-1756-0500-7-160

Cite this article as: Linnerth-Petrik, N.M., Walsh, S.R., Bogner, P.N. et al. BMC Res Notes 2014 7: 160. doi:10.1186-1756-0500-7-160

Abstract

BackgroundAdenocarcinoma is the most common type of non-small cell lung cancer and is frequently observed in non-smoking patients. Adenocarcinoma in-situ formerly referred to as bronchioloalveolar carcinoma is a subset of lung adenocarcinoma characterized by growth along alveolar septae without evidence of stromal, vascular, or pleural invasion, that disproportionately affects never-smokers, women, and Asians. Adenocarcinoma in-situ is morphologically and histologically similar to a contagious lung neoplasm of sheep called ovine pulmonary adenocarcinoma OPA. OPA is caused by infection with the exogenous betaretrovirus, jaagsiekte sheep retrovirus JSRV, whose envelope protein Env is a potent oncogene. Several studies have reported that a proportion of human lung adenocarcinomas are immunopositive for an antigen related to the Gag protein of JSRV, however other groups have been unable to verify these observations by PCR.

MethodsHere we examine human lung cancer tissue arrays TA for evidence of JSRV Env protein and DNA by immunohistochemical staining and PCR, respectively.

ResultsOur results reveal that a subset of human lung cancers express an antigen that reacts with a JSRV Env-specific monoclonal antibody in immunohistochemistry and that exogenous JSRV-like env and gag sequences can be amplified from TA tumor samples, albeit inefficiently.

ConclusionsWhile a causative role has not been established, these data suggest that a JSRV-like virus might infect humans. With next generation sequencing approaches, a JSRV-like virus in human lung cancers may be identified which could have profound implications for prevention, diagnosis and therapy.

KeywordsLung cancer Bronchioloalveolar carcinoma Ovine pulmonary adenocarcinoma Jaagsiekte sheep retrovirus Immunohistochemistry PCR Tissue microarray Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-160 contains supplementary material, which is available to authorized users.

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Autor: Nicolle M Linnerth-Petrik - Scott R Walsh - Paul N Bogner - Carl Morrison - Sarah K Wootton

Fuente: https://link.springer.com/







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