Preparation of a nano emodin transfersome and study on its anti-obesity mechanism in adipose tissue of diet-induced obese ratsReportar como inadecuado

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Journal of Translational Medicine

, 12:72

Nutrition and metabolism


ObjectiveTo describe the preparation of nano emodin transfersome NET and investigate its effect on mRNA expression of adipose triglyceride lipase ATGL and G0-G1 switch gene 2 G0S2 in adipose tissue of diet-induced obese rats.

MethodsNET was prepared by film-ultrasonic dispersion method. The effects of emodin components at different ratios on encapsulation efficiency were investigated.The NET envelopment rate was determined by ultraviolet spectrophotometry. The particle size and Zeta potential of NET were evaluated by Zetasizer analyzer. Sixty male SD rats were assigned to groups randomly. After 8-week treatment, body weight, wet weight of visceral fat and the percentage of body fat PBF were measured. Fasting blood glucose and serum lipid levels were determined. The adipose tissue section was HE stained, and the cellular diameter and quantity of adipocytes were evaluated by light microscopy. The mRNA expression of ATGL and G0S2 from the peri-renal fat tissue was assayed by RT-PCR.

ResultsThe appropriate formulation was deoxycholic acid sodium salt vs. phospholipids 1:8, cholesterol vs. phospholipids 1:3, vitamin Evs. phospholipids 1:20, and emodin vs. phospholipid 1:6. Zeta potential was −15.11 mV, and the particle size was 292.2 nm. The mean encapsulation efficiency was 69.35 ± 0.25%. Compared with the obese model group, body weight, wet weight of visceral fat, PBF and mRNA expression of G0S2 from peri-renal fat tissue were decreased significantly after NET treatment all P < 0.05, while high-density lipoprotein cholesterol HDL-C, the diameter of adipocytes and mRNA expression of ATGL from peri-renal fat tissue were increased significantly all P < 0.05.

ConclusionThe preparation method is simple and reasonable. NET with negative electricity was small and uniform in particle size, with high encapsulation efficiency and stability. NET could reduce body weight and adipocyte size, and this effect was associated with the up-regulation of ATGL, down-regulation of G0S2 expression in the adipose tissue, and improved insulin sensitivity.

KeywordsObesity Nano Emodin Transfersome ATGL G0S2 Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-12-72 contains supplementary material, which is available to authorized users.

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Autor: Kun Lu - Shuanshuan Xie - Shilong Han - Jidong Zhang - Xinwen Chang - Jin Chao - Qingqing Huang - Qing Yuan - Haiyan Lin


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