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BMC Research Notes

, 7:183

First Online: 27 March 2014Received: 09 August 2013Accepted: 17 March 2014DOI: 10.1186-1756-0500-7-183

Cite this article as: Chen, H. & Nalbantoglu, J. BMC Res Notes 2014 7: 183. doi:10.1186-1756-0500-7-183

Abstract

BackgroundAlterations in cell migration are a hallmark of cancer cell invasion and metastasis. In vitro assays commonly used to study cell migration, including the scratch wound healing assay, Boyden chamber assay, and newly developed advanced systems with microfluidics, each have several disadvantages.

FindingsHere we describe an easy and cost-effective in vitro assay for cell migration employing cloning rings to create gaps in the cell monolayer -ring cell migration assay-. The assay was used to quantitate innate differences in cell motility and the effect of various extracellular matrix proteins on migration of five cancer cell lines: U87 and U251N glioma cells, MDA-MB-231and MCF-7 breast cancer cells, and HeLa cervical cancer cells. Interestingly, collagen was a general promoter of cell migration for all five cancer cell lines, without affecting cell proliferation.

ConclusionsTaken together, the ring cell migration assay is an easy, convenient and cost-effective assay to study cell migration in vitro.

KeywordsCell migration assay Cloning ring Extracellular matrices AbbreviationsANOVAAnalysis of variance

ATCCAmerican Type Cell Collection

DMEMDulbecco’s modified Eagle’s medium

EMTEpithelial-to-mesenchymal transition

ECMExtracellular matrix

FBSFetal bovine serum

O.D.Optical density

PBSPhosphate-buffered saline

XTT2,3-Bis-2-Methoxy-4-Nitro-5-Sulfophenyl-2H-Tetrazolium-5-Carboxanilide.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-183 contains supplementary material, which is available to authorized users.

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Autor: Hui Chen - Josephine Nalbantoglu

Fuente: https://link.springer.com/







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