Relevance of the two-component sensor protein CiaH to acid and oxidative stress responses in Streptococcus pyogenesReportar como inadecuado




Relevance of the two-component sensor protein CiaH to acid and oxidative stress responses in Streptococcus pyogenes - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Research Notes

, 7:189

First Online: 28 March 2014Received: 26 September 2013Accepted: 17 March 2014DOI: 10.1186-1756-0500-7-189

Cite this article as: Tatsuno, I., Isaka, M., Okada, R. et al. BMC Res Notes 2014 7: 189. doi:10.1186-1756-0500-7-189

Abstract

BackgroundThe production of virulence proteins depends on environmental factors, and two-component regulatory systems are involved in sensing these factors. We previously established knockout strains in all suspected two-component regulatory sensor proteins of the emm1 clinical strain of S. pyogenes and examined their relevance to acid stimuli in a natural atmosphere. In the present study, their relevance to acid stimuli was re-examined in an atmosphere containing 5% CO2.

ResultsThe spy1236 which is identical to ciaHpy sensor knockout strain showed significant growth reduction compared with the parental strain in broth at pH 6.0, suggesting that the Spy1236 CiaHpy two-component sensor protein is involved in acid response of S. pyogenes. CiaH is also conserved in Streptococcus pneumoniae, and it has been reported that deletion of the gene for its cognate response regulator ciaRpn made the pneumococcal strains more sensitive to oxidative stress. In this report, we show that the spy1236 knockout mutant of S. pyogenes is more sensitive to oxidative stress than the parental strain.

ConclusionsThese results suggest that the two-component sensor protein CiaH is involved in stress responses in S. pyogenes.

AbbreviationsBHI-YBrain-heart infusion yeast

TH-YETodd Hewitt yeast.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-189 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Ichiro Tatsuno - Masanori Isaka - Ryo Okada - Yan Zhang - Tadao Hasegawa

Fuente: https://link.springer.com/







Documentos relacionados