Construction of a high-coverage bacterial artificial chromosome library and comprehensive genetic linkage map of yellowtail Seriola quinqueradiataReport as inadecuate




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BMC Research Notes

, 7:200

First Online: 31 March 2014Received: 13 September 2013Accepted: 26 February 2014DOI: 10.1186-1756-0500-7-200

Cite this article as: Fuji, K., Koyama, T., Kai, W. et al. BMC Res Notes 2014 7: 200. doi:10.1186-1756-0500-7-200

Abstract

BackgroundJapanese amberjack-yellowtail Seriola quinqueradiata is a commonly cultured marine fish in Japan. For cost effective fish production, a breeding program that increases commercially important traits is one of the major solutions. In selective breeding, information of genetic markers is useful and sufficient to identify individuals carrying advantageous traits but if the aim is to determine the genetic basis of the trait, large insert genomic DNA libraries are essential. In this study, toward prospective understanding of genetic basis of several economically important traits, we constructed a high-coverage bacterial artificial chromosome BAC library, obtained sequences from the BAC-end, and constructed comprehensive female and male linkage maps of yellowtail using Simple Sequence Repeat SSR markers developed from the BAC-end sequences and a yellowtail genomic library.

ResultsThe total insert length of the BAC library we constructed here was estimated to be approximately 11 Gb and hence 16-times larger than the yellowtail genome. Sequencing of the BAC-ends showed a low fraction of repetitive sequences comparable to that in Tetraodon and fugu. A total of 837 SSR markers developed here were distributed among 24 linkage groups spanning 1,026.70 and 1,057.83 cM with an average interval of 4.96 and 4.32 cM in female and male map respectively without any segregation distortion. Oxford grids suggested conserved synteny between yellowtail and stickleback.

ConclusionsIn addition to characteristics of yellowtail genome such as low repetitive sequences and conserved synteny with stickleback, our genomic and genetic resources constructed and revealed here will be powerful tools for the yellowtail breeding program and also for studies regarding the genetic basis of traits.

KeywordsYellowtail BAC library Linkage map Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-200 contains supplementary material, which is available to authorized users.

Kanako Fuji, Takashi Koyama contributed equally to this work.

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Author: Kanako Fuji - Takashi Koyama - Wataru Kai - Satoshi Kubota - Kazunori Yoshida - Akiyuki Ozaki - Jun-ya Aoki - Yumi Kawabat

Source: https://link.springer.com/







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