Low-risk susceptibility alleles in 40 human breast cancer cell linesReport as inadecuate




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BMC Cancer

, 9:236

First Online: 16 July 2009Received: 28 October 2008Accepted: 16 July 2009DOI: 10.1186-1471-2407-9-236

Cite this article as: Riaz, M., Elstrodt, F., Hollestelle, A. et al. BMC Cancer 2009 9: 236. doi:10.1186-1471-2407-9-236

Abstract

BackgroundLow-risk breast cancer susceptibility alleles or SNPs confer only modest breast cancer risks ranging from just over 1.0 to1.3 fold. Yet, they are common among most populations and therefore are involved in the development of essentially all breast cancers. The mechanism by which the low-risk SNPs confer breast cancer risks is currently unclear. The breast cancer association consortium BCAC has hypothesized that the low-risk SNPs modulate expression levels of nearby located genes.

MethodsGenotypes of five low-risk SNPs were determined for 40 human breast cancer cell lines, by direct sequencing of PCR-amplified genomic templates. We have analyzed expression of the four genes that are located nearby the low-risk SNPs, by using real-time RT-PCR and Human Exon microarrays.

ResultsThe SNP genotypes and additional phenotypic data on the breast cancer cell lines are presented. We did not detect any effect of the SNP genotypes on expression levels of the nearby-located genes MAP3K1, FGFR2, TNRC9 and LSP1.

ConclusionThe SNP genotypes provide a base line for functional studies in a well-characterized cohort of 40 human breast cancer cell lines. Our expression analyses suggest that a putative disease mechanism through gene expression modulation is not operative in breast cancer cell lines.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-9-236 contains supplementary material, which is available to authorized users.

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Author: Muhammad Riaz - Fons Elstrodt - Antoinette Hollestelle - Abbas Dehghan - Jan GM Klijn - Mieke Schutte

Source: https://link.springer.com/







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