Meiotic maturation of amphibian oocytes increases dephosphorylation of microinjected histones.Reportar como inadecuado




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Resumen

Histone H2b phosphorylated by a cAMP dependent protein kinase was injected into Xenopus laevis oocytes and the hydrolysis of phosphate groups measured. Two hours after injection 60% of the 32p histone radioactivity had been released. Direct microinjection of 32P histone into oocyte nuclei showed that this organelle also has phosphatase activity. 3Hmethylhistone and 125lhistone are not degraded for at least 4 hours in the oocyte. Exposure of oocytes to progesterone for 6 hours to induce meiotic maturation results in a threefold increase in the rate of dephosphorylation. Papaverine and cycloheximide which block maturation prevent this increment in histone phosphatase activity.Nota general

Artículo de publicación ISI



Autor: Allende, Catherine; - Tarragó, Adela; - Allende, Jorge E.; - Seelenfreund Hirsch, Daniela; -

Fuente: http://repositorio.uchile.cl/



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