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Journal of Translational Medicine

, 7:61

First Online: 15 July 2009Received: 29 December 2008Accepted: 15 July 2009DOI: 10.1186-1479-5876-7-61

Cite this article as: Chiriva-Internati, M., Gagliano, N., Donetti, E. et al. J Transl Med 2009 7: 61. doi:10.1186-1479-5876-7-61

Abstract

BackgroundSperm protein 17 Sp17 is a highly conserved mammalian protein characterized in rabbit, mouse, monkey, baboon, macaque, human testis and spermatozoa. mRNA encoding Sp17 has been detected in a range of murine and human somatic tissues. It was also recognized in two myeloma cell lines and in neoplastic cells from patients with multiple myeloma and ovarian carcinoma. These data all indicate that Sp17 is widely distributed in humans, expressed not only in germinal cells and in a variety of somatic tissues, but also in neoplastic cells of unrelated origin.

MethodsSp17 expression was analyzed by immunocytochemistry and transmission electron microscopy on spermatozoa.

ResultsHere, we demonstrate the ultrastructural localization of human Sp17 throughout the spermatozoa flagellar fibrous sheath, and its presence in spermatozoa during in vitro states from their ejaculation to the oocyte fertilization.

ConclusionThese findings suggest a possible role of Sp17 in regulating sperm maturation, capacitation, acrosomal reaction and interactions with the oocyte zona pellucida during the fertilization process. Further, the high degree of sequence conservation throughout its N-terminal half, and the presence of an A-kinase anchoring protein AKAP-binding motif within this region, suggest that Sp17 might play a regulatory role in a protein kinase A-independent AKAP complex in both germinal and somatic cells.

AbbreviationsAKAPskinase anchoring proteins

PKAprotein kinase A

Sp17sperm protein 17

ZPzona pellucida.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-7-61 contains supplementary material, which is available to authorized users.

Maurizio Chiriva-Internati, Nicoletta Gagliano contributed equally to this work.

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Author: Maurizio Chiriva-Internati - Nicoletta Gagliano - Elena Donetti - Francesco Costa - Fabio Grizzi - Barbara Franceschini - El

Source: https://link.springer.com/







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