Expression of ezrin is associated with invasion and dedifferentiation of hepatitis B related hepatocellular carcinomaReport as inadecuate

Expression of ezrin is associated with invasion and dedifferentiation of hepatitis B related hepatocellular carcinoma - Download this document for free, or read online. Document in PDF available to download.

BMC Cancer

, 9:233

First Online: 15 July 2009Received: 01 October 2008Accepted: 15 July 2009DOI: 10.1186-1471-2407-9-233

Cite this article as: Yeh, CN., Pang, ST., Chen, TW. et al. BMC Cancer 2009 9: 233. doi:10.1186-1471-2407-9-233


BackgroundHepatocellular carcinoma HCC is the fifth most common malignancy in the world and constitutes the leading cause of cancer-related death among men, and second among women in Taiwan. Liver cirrhosis and HCC are relatively prevalent, and 80% to 85% of the patients with these conditions have positive results for hepatitis B surface antigen in Taiwan. Only 5% of the general population is seronegative for all hepatititis B virus HBV markers. This is the first study to determine the role of ezrin upon HBV HCC cell and patients with HBV HCC undergoing hepatectomy

MethodsImmunohistochemical study with ezrin in 104 human HBV-HCC cases were carried out to investigate its association with the clinicopathological features and the outcomes of 104 HBV-HCC patients undergoing hepatetomy. In addition, DNA constructs including the wild type ezrin wt-ezrin and mutant ezrin Tyr353 Y353 were transfected into Hep3B cell to study its role in tumor invasion and differentiation.

ResultsHBV HCC patients with ezrin over-expression independently have smaller tumor size, cirrhotic liver background, poor tumor differentiation, and more vascular invasion. Ezrin expression status has no impact on survival for HBV-HCC patients undergoing hepatectomy. The in vitro assay showed that wt-ezrin Hep3B cells have a significant higher level of AFP secretion and higher invasion ability as compared with the control and Y353- ezrin Hep3B cells.

ConclusionEzrin over-expression contributed to de-differentiation and invasion of HBV-HCC cell. HBV-HCC patients with ezrin over-expression were independently associated with tumor with smaller size, cirrhotic liver background, poor differentiation, and vascular invasion.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-9-233 contains supplementary material, which is available to authorized users.

Chun-Nan Yeh, See-Tong Pang contributed equally to this work.

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Author: Chun-Nan Yeh - See-Tong Pang - Tsung-Wen Chen - Ren-Ching Wu - Wen-Hui Weng - Miin-Fu Chen


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