Complement factor H binding by different Lyme disease and relapsing fever Borrelia in animals and humanReport as inadecuate

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BMC Research Notes

, 2:134

First Online: 15 July 2009Received: 06 February 2009Accepted: 15 July 2009DOI: 10.1186-1756-0500-2-134

Cite this article as: Bhide, M.R., Escudero, R., Camafeita, E. et al. BMC Res Notes 2009 2: 134. doi:10.1186-1756-0500-2-134


BackgroundBorreliae employ multiple immune evasive strategies such as binding to complement regulatory proteins factor H fH and factor H like-1 FHL1, differential regulation of surface membrane proteins, antigenic variation, and binding of plasminogen-plasmin and matrix metalloproteinases. As a complement regulatory subunit, fH serves as a cofactor for the factor I-mediated cleavage of C3b. fH binding by Borrelia has been correlated with pathogenesis as well as with host diversity. Here we show the differential binding of borrelial proteins to fH from human and animal sera.

FindingsAffinity ligand binding experiments, 2-D electrophoresis, and protein identification and peptide de novo sequencing based on mass spectrometry, revealed novel fH putative binding proteins of Lyme- and relapsing fever Borrelia. An OspA serotype-associated differential human and animal fH binding by B. garinii was also observed, which could be related with the ability of some strains from serotypes 4 and 7 to invade non-nervous system tissues. Also, the variable affinity of binding proteins expressed by different Borrelia to animal fH correlated with their host selectivity.

ConclusionThe novel animal and human putative fH binding proteins FHBPs in this study underscore the importance of evasion of complement in the pathogenesis of Borrelia infections.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-2-134 contains supplementary material, which is available to authorized users.

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Author: Mangesh R Bhide - Raquel Escudero - Emilio Camafeita - Horacio Gil - Isabel Jado - Pedro Anda


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