Association of a single nucleotide polymorphism in akirin 2 gene with marbling in Japanese Black beef cattleReport as inadecuate




Association of a single nucleotide polymorphism in akirin 2 gene with marbling in Japanese Black beef cattle - Download this document for free, or read online. Document in PDF available to download.

BMC Research Notes

, 2:131

First Online: 14 July 2009Received: 27 May 2009Accepted: 14 July 2009DOI: 10.1186-1756-0500-2-131

Cite this article as: Sasaki, S., Yamada, T., Sukegawa, S. et al. BMC Res Notes 2009 2: 131. doi:10.1186-1756-0500-2-131

Abstract

BackgroundMarbling defined by the amount and distribution of intramuscular fat, so-called Shimofuri, is an economically important trait of beef cattle in Japan. The c17-25 expressed sequence tag EST has been previously shown to possess expression difference in musculus longissimus muscle between low-marbled and high-marbled steer groups, and to be located within genomic region of a quantitative trait locus for marbling. Thus, the akirin 2 AKIRIN2 gene containing the c17-25 EST sequence was considered as a positional functional candidate for the gene responsible for marbling. In this study, we explored single nucleotide polymorphism SNP in the AKIRIN2 and analyzed association of the SNP with marbling.

FindingsA SNP in the 3- untranslated region of the AKIRIN2, referred to as c.*188G>A, was the only difference detected between high- and low-marbled steer groups. The SNP was associated with marbling in 3 experiments using 100 sires P = 0.041, 753 paternal half-sib progeny steers from 4 sires heterozygous for the c.*188G>A P = 0.005, and 730 paternal half-sib progeny steers from 3 sires homozygous for the A allele at the c.*188G>A P = 0.047, in Japanese Black beef cattle. The effect of genotypes of the SNP on subcutaneous fat thickness was not statistically significant P > 0.05.

ConclusionThese findings suggest that the AKIRIN2 SNP polymorphism is associated with marbling and may be useful for effective marker-assisted selection to increase the levels of marbling in Japanese Black beef cattle.

Seiki Sasaki, Takahisa Yamada, Shin Sukegawa contributed equally to this work.

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Author: Seiki Sasaki - Takahisa Yamada - Shin Sukegawa - Takeshi Miyake - Tatsuo Fujita - Mitsuo Morita - Takeshi Ohta - Youichi T

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