Inhibition of tumoral cell respiration and growth by nordihydroguaiaretic acidReport as inadecuate

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The effects of nordihydroguaiaretic acid NDGA, best known as an inhibitor of lipoxygenase activities, on the culture growth, oxygen consumption, ATP level, viability, and redox state of some electron carriers of intact TA3 and 786A ascites tumor cells have been studied. NDGA inhibited the respiration rate of these two tumor cell lines by preventing electron flow through the respiratory chain. Consequently, ATP levels, cell viability and culture growth rates were decreased. NDGA did not noticeably inhibit electron flow through both cytochrome oxidase and ubiquinone-cytochrome tie, complex. Also, the presence of NDGA changed to redox state of NADP+ to a more reduced level, and the redox states of ubiquinone, cytochrome b and cytochromes c + c1 changed to a more oxidized level. These observations suggest that the electron transport in the tumor mitochondria was inhibited by NDGA at the NADH-dehydrogenase-ubiquinone level energy-conserving site 1. As a consequence, mitochondrial ATP synthesis would be interrupted. This event could be related to the cytotoxic effect of NDGA.Nota general

Artículo de publicación ISI

Author: Pavani, M.; - Ferreira Parker, Jorge; - Fones, E.; - Oksenberg Reisberg, Dan; - García, M.; - Hernández, C.; - Cordano, G.; - M



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