Comparative expression profiles of microRNA in left and right atrial appendages from patients with rheumatic mitral valve disease exhibiting sinus rhythm or atrial fibrillationReport as inadecuate




Comparative expression profiles of microRNA in left and right atrial appendages from patients with rheumatic mitral valve disease exhibiting sinus rhythm or atrial fibrillation - Download this document for free, or read online. Document in PDF available to download.

Journal of Translational Medicine

, 12:90

Cardiovascular, Metabolic and Lipoprotein Translation

Abstract

BackgroundThe atrial fibrillation AF associated microRNAs miRNAs were found in the right atrium RA and left atrium LA from patients with rheumatic mitral valve disease RMVD. However, most studies only focus on the RA; and the potential differences of AF-associated miRNAs between the RA and LA are still unknown. The aim of this study was to perform miRNA expression profiles analysis to compare the potential differences of AF-associated miRNAs in the right atrial appendages RAA and left atrial appendages LAA from RMVD patients.

MethodsSamples tissues from the RAA and LAA were obtained from 18 RMVD patients 10 with AF during mitral valve replacement surgery. From these tissues, miRNA expression profiles were created and analyzed using a human miRNA microarray. Then, the results were validated using qRT-PCR analysis for 12 selected miRNAs. Finally, potential targets of 10 validated miRNAs were predicted and their functions and potential pathways were analyzed using the miRFocus database.

ResultsIn RAA, 65 AF-associated miRNAs were found and significantly dysregulated i.e. 28 miRNAs were up-regulated and 37 were down-regulated. In LAA, 42 AF-associated miRNAs were found and significantly dysregulated i.e. 22 miRNAs were up-regulated and 20 were down-regulated. Among these AF-associated miRNAs, 23 of them were found in both RAA and LAA, 45 of them were found only in RAA, and 19 of them were found only in LAA. Finally, 10 AF-associated miRNAs validated by qRT-PCR were similarly distributed in RAA and LAA; 3 were found in both RAA and LAA, 5 were found only in RAA, and 2 were found only in LAA. Potential miRNA targets and molecular pathways were identified.

ConclusionsWe have found the different distributions of AF-associated miRNAs in the RAA and LAA from RMVD patients. This may reflect different miRNA mechanisms in AF between the RA and LA. These findings may provide new insights into the underlying mechanisms of AF in RMVD patients.

KeywordsAtrial fibrillation microRNA Rheumatic mitral valve disease AbbreviationsAFAtrial fibrillation

SRSinus rhythm

RMVDRheumatic mitral valve disease

MiRNAmicroRNA

RARight atrium

LALeft atrium

RAARight atrial appendage

LAALeft atrial appendage

ECGElectrocardiogram

NYHANew York Heart Association

LVEFLeft ventricular ejection fraction

PGRPhotogene.rated reagent

CVCoefficient of variation

qRT-PCRQuantitative real-time PCR

GOGene Ontology

KEGGKyoto Encyclopedia of Genes and Genomes.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-12-90 contains supplementary material, which is available to authorized users.

Hai Liu, Han Qin, Guang-xian Chen contributed equally to this work.

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Author: Hai Liu - Han Qin - Guang-xian Chen - Meng-ya Liang - Jian Rong - Jian-ping Yao - Zhong-kai Wu

Source: https://link.springer.com/







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