Activities of cardiac tissue matrix metalloproteinases 2 and 9 are reduced by remote ischemic preconditioning in cardiosurgical patients with cardiopulmonary bypassReport as inadecuate




Activities of cardiac tissue matrix metalloproteinases 2 and 9 are reduced by remote ischemic preconditioning in cardiosurgical patients with cardiopulmonary bypass - Download this document for free, or read online. Document in PDF available to download.

Journal of Translational Medicine

, 12:94

Cardiovascular, Metabolic and Lipoprotein Translation

Abstract

BackgroundTransient episodes of ischemia in a remote organ or tissue remote ischemic preconditioning, RIPC can attenuate myocardial injury. Myocardial damage is associated with tissue remodeling and the matrix metalloproteinases 2 and 9 MMP-2-9 are crucially involved in these events. Here we investigated the effects of RIPC on the activities of heart tissue MMP-2-9 and their correlation with serum concentrations of cardiac troponin T cTnT, a marker for myocardial damage.

MethodsIn cardiosurgical patients with cardiopulmonary bypass CPB RIPC was induced by four 5 minute cycles of upper limb ischemia-reperfusion. Cardiac tissue was obtained before as well as after CPB and serum cTnT concentrations were measured. Tissue derived from control patients N = 17 with high cTnT concentrations ≥0.32 ng-ml and RIPC patients N = 18 with low cTnT ≤0.32 ng-ml was subjected to gelatin zymography to quantify MMP-2-9 activities.

ResultsIn cardiac biopsies obtained before CPB, activities of MMP-2-9 were attenuated in the RIPC group MMP-2: Control, 1.13 ± 0.13 a.u.; RIPC, 0.71 ± 0.12 a.u.; P < 0.05. MMP-9: Control, 1.50 ± 0.16 a.u.; RIPC, 0.87 ± 0.14 a.u.; P < 0.01, while activities of the pro-MMPs were not altered P > 0.05. In cardiac biopsies taken after CPB activities of pro- and active MMP-2-9 were not different between the groups P > 0.05. Spearman’s rank tests showed that MMP-2-9 activities in cardiac tissue obtained before CPB were positively correlated with postoperative cTnT serum levels MMP-2, P = 0.016; MMP-9, P = 0.015.

ConclusionsActivities of MMP-2-9 in cardiac tissue obtained before CPB are attenuated by RIPC and are positively correlated with serum concentrations of cTnT. MMPs may represent potential targets for RIPC mediated cardioprotection.

Trial registrationClinicalTrials.gov identifierNCT00877305.

KeywordsCardioprotection Ischemia-reperfusion injury Matrix metalloproteinases Myocardial damage Remote ischemic preconditioning Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-12-94 contains supplementary material, which is available to authorized users.

Karina Zitta, Patrick Meybohm contributed equally to this work.

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Author: Karina Zitta - Patrick Meybohm - Berthold Bein - Matthias Gruenewald - Fabian Lauer - Markus Steinfath - Jochen Cremer - Ka

Source: https://link.springer.com/







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