Staphylococcal enterotoxin B suppresses Alix and compromises intestinal epithelial barrier functionsReportar como inadecuado




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Journal of Biomedical Science

, 21:29

First Online: 09 April 2014Received: 28 October 2013Accepted: 02 April 2014DOI: 10.1186-1423-0127-21-29

Cite this article as: Yan, H., Yi, H., Xia, L. et al. J Biomed Sci 2014 21: 29. doi:10.1186-1423-0127-21-29

Abstract

BackgroundThe epithelial barrier dysfunction plays a critical role in the pathogenesis of a broad array of immune diseases. Alix protein is involved in the endolysosome system. This study aims to elucidate the role of Alix in the maintenance of epithelial barrier function.

ResultsThe results showed that Alix was detected in T84 cells at both mRNA and protein levels. Exposure to Staphylococcal enterotoxin B SEB markedly suppressed the expression of Alix in T84 cells, which could be blocked by knocking down the Toll like receptor 2. The exposure to SEB did not affect the TER, but markedly increased the permeability of T84 monolayers to OVA; the OVA passing through T84 monolayers still preserved the antigenicity manifesting inducing antigen specific T cells proliferation.

ConclusionsAlix protein plays a critical role in the maintenance of the barrier function of T84 monolayers.

KeywordsAlix Epithelial cell Barrier function Staphylococcal enterotoxin B Antigenicity Electronic supplementary materialThe online version of this article doi:10.1186-1423-0127-21-29 contains supplementary material, which is available to authorized users.

Hao Yan, Haitao Yi, Lixin Xia contributed equally to this work.

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Autor: Hao Yan - Haitao Yi - Lixin Xia - Zhenke Zhan - Weiyi He - Jijuan Cao - Ping-Chang Yang - Zhigang Liu

Fuente: https://link.springer.com/







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