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BMC Research Notes

, 2:125

First Online: 07 July 2009Received: 04 February 2009Accepted: 07 July 2009DOI: 10.1186-1756-0500-2-125

Cite this article as: Heider, D., Pyka, M. & Barnekow, A. BMC Res Notes 2009 2: 125. doi:10.1186-1756-0500-2-125

Abstract

BackgroundDNA watermarks can be applied to identify the unauthorized use of genetically modified organisms. It has been shown that coding regions can be used to encrypt information into living organisms by using the DNA-Crypt algorithm. Yet, if the sequence of interest presents a non-coding DNA sequence, either the function of a resulting functional RNA molecule or a regulatory sequence, such as a promoter, could be affected. For our studies we used the small cytoplasmic RNA 1 in yeast and the lac promoter region of Escherichia coli.

FindingsThe lac promoter was deactivated by the integrated watermark. In addition, the RNA molecules displayed altered configurations after introducing a watermark, but surprisingly were functionally intact, which has been verified by analyzing the growth characteristics of both wild type and watermarked scR1 transformed yeast cells. In a third approach we introduced a second overlapping watermark into the lac promoter, which did not affect the promoter activity.

ConclusionEven though the watermarked RNA and one of the watermarked promoters did not show any significant differences compared to the wild type RNA and wild type promoter region, respectively, it cannot be generalized that other RNA molecules or regulatory sequences behave accordingly. Therefore, we do not recommend integrating watermark sequences into regulatory regions.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-2-125 contains supplementary material, which is available to authorized users.

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Autor: Dominik Heider - Martin Pyka - Angelika Barnekow

Fuente: https://link.springer.com/







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