Comprehensive proteomic analysis of white blood cells from chikungunya fever patients of different severitiesReportar como inadecuado




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Journal of Translational Medicine

, 12:96

Immunovirology

Abstract

BackgroundChikungunya fever CHIKF is a recently re-emerged mosquito transmitted viral disease caused by the chikungunya virus CHIKV, an Alphavirus belonging to the family Togaviridae. Infection of humans with CHIKV can result in CHIKF of variable severity, although the factors mediating disease severity remain poorly defined.

MethodsWhite blood cells were isolated from blood samples collected during the 2009-2010 CHIKF outbreak in Thailand. Clinical presentation and viral load data were used to classify samples into three groups, namely non chikungunya fever non-CHIKF, mild CHIKF, and severe CHIKF. Five samples from each group were analyzed for protein expression by GeLC-MS-MS.

ResultsCHIKV proteins structural and non-structural were found only in CHIKF samples. A total of 3505 human proteins were identified, with 68 proteins only present in non-CHIKF samples. A total of 240 proteins were found only in CHIKF samples, of which 65 and 46 were found only in mild and severe CHIKF samples respectively. Proteins with altered expression mapped predominantly to cellular signaling pathways including toll-like receptor and PI3K-Akt signaling although many other processes showed altered expression as a result of CHIKV infection. Expression of proteins consistent with the activation of the inflammasome was detected, and quantitation of pro-caspase 1 at the protein and RNA levels showed an association with disease severity.

ConclusionsThis study confirms the infection of at least a component of white blood cells by CHIKV, and shows that CHIKV infection results in activation of the inflammasome in a manner that is associated with disease severity.

KeywordsChikungunya Proteome Inflammasome Caspase 1 Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-12-96 contains supplementary material, which is available to authorized users.

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Autor: Nitwara Wikan - Sarawut Khongwichit - Weerawat Phuklia - Sukathida Ubol - Tipparat Thonsakulprasert - Montri Thannagith - Du

Fuente: https://link.springer.com/







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