The expression of Dicer and Drosha in matched normal tissues, tumours and lymph node metastases in triple negative breast cancerReport as inadecuate




The expression of Dicer and Drosha in matched normal tissues, tumours and lymph node metastases in triple negative breast cancer - Download this document for free, or read online. Document in PDF available to download.

BMC Cancer

, 14:253

Translational oncology

Abstract

BackgroundBreast cancer is the most common malignancy in women world-wide. Triple negative breast cancer TNBC is a highly aggressive subtype that lacks expression of hormone receptors for estrogen, progesterone and human epidermal growth factor 2; and is associated with a high propensity for metastatic spread. Several studies have identified critical roles for microRNAs in breast cancer, but the role of two critical enzymes involved in microRNA biogenesis, Dicer and Drosha, is not well understood, particularly with respect to metastatic progression in this subtype.

MethodsWe examined the expression of Dicer and Drosha in a series of invasive 35 TNBCs with matched normal adjacent tissues n = 18 and lymph node metastases n = 15 using semi-quantitative real time RT-PCR. The relationship of their expression with clinical features including age at diagnosis, lymph node positivity and tumour size was analysed.

ResultsWe report that Dicer was significantly decreased while Drosha was significantly increased in tumours when compared to normal adjacent tissues. While there was no difference in Drosha expression in lymph node metastases when compared to the primary tumour, Dicer was significantly increased. There was no correlation between the expression of either Dicer or Drosha to age at diagnosis, lymph node positivity and tumour size.

ConclusionsIn conclusion, Dicer and Drosha are dysregulated in TNBC and matched lymph node metastases however, the clinical relevance of this is still not known. The altered expression of Dicer and Drosha may serve as markers for disrupted miRNA biogenesis in TNBC.

KeywordsDicer Drosha Breast cancer Metastasis Triple negative AbbreviationsTNBCTriple negative breast cancer

miRNAmicroRNA

RT-PCRReverse transcription polymerase chain reaction

EREstrogen receptor

PRProgesterone receptor

HER2Human epidermal growth factor receptor

LNLymph node

NATNormal adjacent tissue

IDCInvasive ductal carcinoma

FFPEFormalin fixed paraffin embedded

RNARibonucleic acid

cDNAcomplementary deoxyribonucleic acid.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-14-253 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Kelly A Avery-Kiejda - Stephen G Braye - John F Forbes - Rodney J Scott

Source: https://link.springer.com/







Related documents