Ultraviolet-ozone treatment reduces levels of disease-associated prion protein and prion infectivityReportar como inadecuado




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BMC Research Notes

, 2:121

Prions

Abstract

BackgroundTransmissible spongiform encephalopathies TSEs are a group of fatal neurodegenerative diseases caused by novel infectious agents referred to as prions. Prions appear to be composed primarily, if not exclusively, of a misfolded isoform of the cellular prion protein. TSE infectivity is remarkably stable and can resist many aggressive decontamination procedures, increasing human, livestock and wildlife exposure to TSEs.

FindingsWe tested the hypothesis that UV-ozone treatment reduces levels of the pathogenic prion protein and inactivates the infectious agent. We found that UV-ozone treatment decreased the carbon and prion protein content in infected brain homogenate to levels undetectable by dry-ashing carbon analysis or immunoblotting, respectively. After 8 weeks of ashing, UV-ozone treatment reduced the infectious titer of treated material by a factor of at least 10. A small amount of infectivity, however, persisted despite UV-ozone treatment. When bound to either montmorillonite clay or quartz surfaces, PrP was still susceptible to degradation by UV-ozone.

ConclusionOur findings strongly suggest that UV-ozone treatment can degrade pathogenic prion protein and inactivate prions, even when the agent is associated with surfaces. Using larger UV-ozone doses or combining UV-ozone treatment with other decontaminant methods may allow the sterilization of TSE-contaminated materials.

AbbreviationsBHbrain homogenate

dpidays post-inoculation

HYHyper strain of hamster-passaged transmissible mink encephalopathy agent

Mtemontmorillonite clay

PAGEpolyacrylamide gel electrophoresis

PBSphosphate buffered saline

PKproteinase K

PrPcellular prion protein

PrPprion protein

PrPdisease-associated prion protein

TSEtransmissible spongiform encephalopathy

UVultraviolet.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-2-121 contains supplementary material, which is available to authorized users.

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Autor: Christopher J Johnson - PUPA Gilbert - Debbie McKenzie - Joel A Pedersen - Judd M Aiken

Fuente: https://link.springer.com/







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