Quaternized chitosan as support for the assembly of gold nanoparticles and glucose oxidase: Physicochemical characterization of the platform and evaluation of its biocatalytic activityReport as inadecuate




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We report for the first time the use of quaternized chitosan QCHI for the immobilization of gold nanopartides NP and glucose oxidase GOD, the characterization of the resulting platform and its biocatalytic activity using glucose as substrate. The chemical substitution of chitosan has allowed us to work at physiologic pH to build up self-assembled layers of QCHI-NP as platform for the enzyme immobilization. The adsorption of GOD was analyzed by surface plasmon resonance SPR to compare the surface coverage of GOD in absence and presence of the QCHI-NP platform. The results obtained with cyclic voltammetry and scanning electrochemical microscopy SECM revealed that the adsorption of NP improves the conductivity of the structure and its electrochemical reactivity, facilitating the oxidation of the hydrogen peroxide produced by GOD. The electrodes modified with NP present higher amperometric response demonstrating the efficient transduction of the enzymatic activity in this structure.Nota general

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Author: Bracamonte, María V.; - Bollo Dragnic, Soledad; - Labbé, Pierre; - Rivas, Gustavo A.; - Ferreyra, Nancy F.; -

Source: http://repositorio.uchile.cl/



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