Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA studyReportar como inadecuado

Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA study - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Cancer

, 14:269

Translational oncology


BackgroundThe receptor for urokinase-type plasminogen activator uPAR is associated with cancer development and progression. Within the tumor microenvironment uPAR is expressed by malignant cells as well as tumor-associated stromal cells. However, the contribution of uPAR expression in these stromal cells to malignancy and patient survival in colorectal cancer is still unclear. This study compares the association of uPAR expression in both colorectal tumor-associated stromal cells and neoplastic cells with clinico-pathological characteristics and patient survival using tissue micro arrays TMA.

MethodsImmunohistochemical staining of uPAR expression was performed on tumor tissue from 262 colorectal cancer patients. Kaplan-Meier, log rank, and uni- and multivariate Cox’s regression analyses were used to calculate associations between uPAR expression and patient survival.

ResultsIn the colorectal tumor-associated stromal microenvironment, uPAR is expressed in macrophages, neoangiogenic endothelial cells and myofibroblasts. uPAR expression in tumor-associated stromal cells and neoplastic cells and both combined were negatively associated with overall survival OS and Disease Free Survival DFS. Uni- and multivariate Cox’s regression analysis for combined uPAR expression in tumor-associated stromal and neoplastic cells showed significant and independent negative associations with OS and DFS. Only uPAR expression in tumor-associated stromal cells showed independent significance in the uni- and multivariate analysis for DFS.

ConclusionThis study demonstrates a significant independent negative association between colorectal cancer patient survival and uPAR expression in especially tumor-associated stromal cells.

KeywordsUrokinase receptor Immunohistochemistry Diagnosis Survival Tumor associated stromal cell Macrophage AbbreviationsuPARurokinase-type Plasminogen Activator Receptor

suPARsoluble urokinase-type Plasminogen Activator Receptor

uPAurokinase-type Plasminogen Activator

ECMExtracellular matrix

TNMTumor nodes metastasis

HRHazard ratio

OSOverall survival

DFSDisease free survival

TMATissue microarray


MSIMicrosatellite instability

MSSMicrosatellite stable.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-14-269 contains supplementary material, which is available to authorized users.

Martin C Boonstra, Floris PR Verbeek contributed equally to this work.

Download fulltext PDF

Autor: Martin C Boonstra - Floris PR Verbeek - Andrew P Mazar - Hendrica AJM Prevoo - Peter JK Kuppen - Cornelis JH van de Ve


Documentos relacionados