Differential gene expression signatures for cell wall integrity found in chitin synthase II chs2 Δ and myosin II myo1 Δ deficient cytokinesis mutants of Saccharomyces cerevisiaeReportar como inadecuado




Differential gene expression signatures for cell wall integrity found in chitin synthase II chs2 Δ and myosin II myo1 Δ deficient cytokinesis mutants of Saccharomyces cerevisiae - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Research Notes

, 2:87

First Online: 09 May 2009Received: 16 March 2009Accepted: 09 May 2009DOI: 10.1186-1756-0500-2-87

Cite this article as: Rodríguez-Quiñones, J.F. & Rodríguez-Medina, J.R. BMC Res Notes 2009 2: 87. doi:10.1186-1756-0500-2-87

Abstract

BackgroundMyosin II-dependent contraction of the cytokinetic ring and primary septum formation by chitin synthase II are interdependent processes during cytokinesis in Saccharomyces cerevisiae. Hence, null mutants of myosin II myo1 Δ and chitin synthase II chs2 Δ share multiple morphological and molecular phenotypes. To understand the nature of their interdependent functions, we will seek to identify genes undergoing transcriptional regulation in chs2 Δ strains and to establish a transcription signature profile for comparison with myo1 Δ strains.

ResultsA total of 467 genes were commonly regulated between myo1Δ and chs2Δ mutant strains p ≤ 0.01. Common regulated biological process categories identified by Gene Set Enrichment Analysis GSEA in both gene expression profiles were: protein biosynthesis, RNA processing, and stress response. Expression of 17-20 genes in the main transcriptional fingerprint for cell wall stress was confirmed in the chs2Δ strain versus 5-20 for the myo1Δ strain. One of these genes, SLT2-MPK1, was up-regulated in both strains and both strains accumulated the hyperphosphorylated form of Slt2p thereby confirming that the PKC1 cell wall integrity pathway CWIP was activated by both mutations. The SLT2-MPK1 gene, essential for myo1Δ strains, was not required in the chs2Δ strain.

ConclusionComparison of the chs2Δ and myo1 Δ gene expression profiles revealed similarities in the biological process categories that respond to the chs2Δ and myo1 Δ gene mutations. This supports the view that these mutations affect a common function in cytokinesis. Despite their similarities, these mutants exhibited significant differences in expression of the main transcriptional fingerprint for cell wall stress and their requirement of the CWIP for survival.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-2-87 contains supplementary material, which is available to authorized users.

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Autor: José F Rodríguez-Quiñones - José R Rodríguez-Medina

Fuente: https://link.springer.com/







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