The proximal first exon architecture of the murine ghrelin gene is highly similar to its human orthologueReportar como inadecuado




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BMC Research Notes

, 2:85

First Online: 09 May 2009Received: 12 January 2009Accepted: 09 May 2009DOI: 10.1186-1756-0500-2-85

Cite this article as: Seim, I., Carter, S.L., Herington, A.C. et al. BMC Res Notes 2009 2: 85. doi:10.1186-1756-0500-2-85

Abstract

BackgroundThe murine ghrelin gene Ghrl, originally sequenced from stomach tissue, contains five exons and a single transcription start site in a short, 19 bp first exon exon 0. We recently isolated several novel first exons of the human ghrelin gene and found evidence of a complex transcriptional repertoire. In this report, we examined the 5- exons of the murine ghrelin orthologue in a range of tissues using 5- RACE.

Findings5- RACE revealed two transcription start sites TSSs in exon 0 and four TSSs in intron 0, which correspond to 5- extensions of exon 1. Using quantitative, real-time RT-PCR qRT-PCR, we demonstrated that extended exon 1 containing Ghrl transcripts are largely confined to the spleen, adrenal gland, stomach, and skin.

ConclusionWe demonstrate that multiple transcription start sites are present in exon 0 and an extended exon 1 of the murine ghrelin gene, similar to the proximal first exon organisation of its human orthologue. The identification of several transcription start sites in intron 0 of mouse ghrelin resulting in an extension of exon 1 raises the possibility that developmental-, cell- and tissue-specific Ghrl mRNA species are created by employing alternative promoters and further studies of the murine ghrelin gene are warranted.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-2-85 contains supplementary material, which is available to authorized users.

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Autor: Inge Seim - Shea L Carter - Adrian C Herington - Lisa K Chopin

Fuente: https://link.springer.com/







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