Expression of 5S rDNA in the oocytes of water frogsReportar como inadecuado




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BMC Research Notes

, 2:10

Quantitative real time PCR normalization and optimization

Abstract

BackgroundWe report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR. This kind of research had never been performed before.

Results5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes. The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

ConclusionWe suggest the possibility of using 5S rDNA as the internal control gene, in the studies of relative mRNA quantitative assays in water frog oocytes, because of its characteristic specific expression pattern in the Rana lessonae, Rana ridibunda and Rana esculenta oocytes.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-2-10 contains supplementary material, which is available to authorized users.

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Autor: Elżbieta Czarniewska - Robert Plewa

Fuente: https://link.springer.com/







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