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Reference: Birts, CN, Sanzone, AP, El-Sagheer, AH et al., (2014). Transcription of click-linked DNA in human cells. Angewandte Chemie (International ed. in English), 53 (9), 2362-2365.Citable link to this page:


Transcription of click-linked DNA in human cells.

Abstract: Click DNA ligation promises an alternative to the current enzymatic approaches for DNA assembly, with the ultimate goal of using efficient chemical reactions for the total chemical synthesis and assembly of genes and genomes. Such an approach would enable the incorporation of various chemically modified bases throughout long stretches of DNA, a feat not possible with current polymerase-based methods. An unequivocal requirement for this approach is the biocompatibility of the resulting triazole-linked DNA. The correct function of this unnatural DNA linker in human cells is demonstrated here by using a click-linked gene encoding the fluorescent protein mCherry. Reverse transcription of mRNA isolated from these cells and subsequent sequencing of the mCherry cDNA shows error-free transcription. Nucleotide excision repair (NER) is shown to not play a role in the observed biocompatibility by using a NER-deficient human cell line. This is the first example of a non-natural DNA linker being functional in a eukaryotic cell.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Cancer Research UK   Funder: Breast Cancer Campaign   Funder: Biotechnology and Biological Sciences Research Council   Notes:Copyright © 2014 The Authors. Published by Wiley-VCH Verlag GmbH and Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Bibliographic Details

Publisher: Wiley-VCH Verlag

Publisher Website:

Journal: Angewandte Chemie (International ed. in English)see more from them

Publication Website:

Issue Date: 2014-02


Urn: uuid:2fe26a2c-c0ce-4e99-a5bb-b40a91b243b8

Source identifier: 446931

Eissn: 1521-3773


Issn: 1433-7851 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Cell Line Humans Triazoles Luminescent Proteins DNA DNA Repair Transcription, Genetic Click Chemistry Synthetic Biology Tiny URL: pubs:446931


Autor: Birts, CN - - - Sanzone, AP - - - El-Sagheer, AH - - - Blaydes, JP - - - Brown, T - institutionUniversity of Oxford - - - Tavasso



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