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Reference: Smith, TK, Vasileva, N, Gluenz, E et al., (2009). Blocking variant surface glycoprotein synthesis in Trypanosoma brucei triggers a general arrest in translation initiation. PloS one, 4 (10), Article: e7532.Citable link to this page:

 

Blocking variant surface glycoprotein synthesis in Trypanosoma brucei triggers a general arrest in translation initiation.

Abstract: BACKGROUND: The African trypanosome Trypanosoma brucei is covered with a dense layer of Variant Surface Glycoprotein (VSG), which protects it from lysis by host complement via the alternative pathway in the mammalian bloodstream. Blocking VSG synthesis by the induction of VSG RNAi triggers an unusually precise precytokinesis cell-cycle arrest. METHODOLOGY/PRINCIPAL FINDINGS: Here, we characterise the cells arrested after the induction of VSG RNAi. We were able to rescue the VSG221 RNAi induced cell-cycle arrest through expression of a second different VSG (VSG117 which is not recognised by the VSG221 RNAi) from the VSG221 expression site. Metabolic labeling of the arrested cells showed that blocking VSG synthesis triggered a global translation arrest, with total protein synthesis reduced to less than 1-4% normal levels within 24 hours of induction of VSG RNAi. Analysis by electron microscopy showed that the translation arrest was coupled with rapid disassociation of ribosomes from the endoplasmic reticulum. Polysome analysis showed a drastic decrease in polysomes in the arrested cells. No major changes were found in levels of transcription, total RNA transcript levels or global amino acid concentrations in the arrested cells. CONCLUSIONS: The cell-cycle arrest phenotype triggered by the induction of VSG221 RNAi is not caused by siRNA toxicity, as this arrest can be alleviated if a second different VSG is inserted downstream of the active VSG221 expression site promoter. Analysis of polysomes in the stalled cells showed that the translation arrest is mediated at the level of translation initiation rather than elongation. The cell-cycle arrest induced in the presence of a VSG synthesis block is reversible, suggesting that VSG synthesis and/or trafficking to the cell surface could be monitored during the cell-cycle as part of a specific cell-cycle checkpoint.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Wellcome Trust   Notes:Copyright 2009 Smith et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website: http://www.plos.org

Journal: PloS onesee more from them

Publication Website: http://www.plosone.org

Issue Date: 2009

pages:Article: e7532Identifiers

Urn: uuid:3d6e8e30-4634-455c-bb24-d24c89673e9a

Source identifier: 9749

Eissn: 1932-6203

Doi: https://doi.org/10.1371/journal.pone.0007532

Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Animals Endoplasmic Reticulum Polyribosomes Trypanosoma brucei brucei Trypanosomiasis, African Nucleic Acids Glycoproteins Amino Acids Variant Surface Glycoproteins, Trypanosoma Phenotype Transcription, Genetic RNA Interference Genetic Variation Cell Cycle Protein Biosynthesis Tiny URL: pubs:9749

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Autor: Smith, TK - - - Vasileva, N - institutionUniversity of Oxford - - - Gluenz, E - institutionUniversity of Oxford Oxford, MSD, Path

Fuente: https://ora.ox.ac.uk/objects/uuid:3d6e8e30-4634-455c-bb24-d24c89673e9a



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