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Reference: Woodhouse, SD, Narayan, R, Latham, S et al., (2010). Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro. Hepatology, 52 (2), 443-453.Citable link to this page:

 

Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro.

Abstract: Hepatitis C virus (HCV) is a major cause of liver disease but the full impact of HCV infection on the hepatocyte is poorly understood. RNA sequencing (RNA-Seq) is a novel method to analyze the full transcriptional activity of a cell or tissue, thus allowing new insight into the impact of HCV infection. We conducted the first full-genome RNA-Seq analysis in a host cell to analyze infected and noninfected cells, and compared this to microarray and proteomic analyses. The combined power of the triple approach revealed that HCV infection affects a number of previously unreported canonical pathways and biological functions, including pregnane X receptor/retinoic acid receptor activation as a potential host antiviral response, and integrin-linked kinase signaling as an entry factor. This approach also identified several mechanisms implicated in HCV pathogenesis, including an increase in reactive oxygen species. HCV infection had a broad effect on cellular metabolism, leading to increases in cellular cholesterol and free fatty acid levels, associated with a profound and specific decrease in cellular glucose levels.RNA-Seq technology, especially when combined with established methods, demonstrated that HCV infection has potentially wide-ranging effects on cellular gene and protein expression. This in vitro study indicates a substantial metabolic impact of HCV infection and highlights new mechanisms of virus-host interaction which may be highly relevant to pathogenesis in vivo.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Wellcome Trust   Funder: National Institutes of Health   Notes:Copyright © 2010 by the American Association for the Study of Liver Disease. Published by Wiley on behalf of the American Association for the Study of Liver Disease. Open Access through the Online Open option. This is the publisher's version of the article. The final version is available online from Wiley at: [10.1002/hep.23733]

Bibliographic Details

Publisher: Wiley

Publisher Website: http://eu.wiley.com/WileyCDA/

Journal: Hepatologysee more from them

Publication Website: http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1527-3350

Issue Date: 2010-04-23

pages:443-453Identifiers

Urn: uuid:43d1df2a-9020-4074-bf51-0af0277c37d3

Source identifier: 66095

Eissn: 1527-3350

Doi: https://doi.org/10.1002/hep.23733

Issn: 0270-9139 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Cells, Cultured Hepatitis C Proteome Microarray Analysis Gene Expression Profiling Tiny URL: pubs:66095

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Autor: Woodhouse, SD - institutionUniversity of Oxford Oxford, MSD, Biochemistry - - - Narayan, R - institutionUniversity of Oxford Oxfo

Fuente: https://ora.ox.ac.uk/objects/uuid:43d1df2a-9020-4074-bf51-0af0277c37d3



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