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Reference: Oliver, PL, Chodroff, RA, Gosal, A et al., (2014). Disruption of Visc-2, a Brain-Expressed Conserved Long Noncoding RNA, Does Not Elicit an Overt Anatomical or Behavioral Phenotype. Cerebral cortex (New York, N.Y. : 1991).Citable link to this page:

 

Disruption of Visc-2, a Brain-Expressed Conserved Long Noncoding RNA, Does Not Elicit an Overt Anatomical or Behavioral Phenotype.

Abstract: Although long noncoding RNAs (lncRNAs) are proposed to play essential roles in mammalian neurodevelopment, we know little of their functions from their disruption in vivo. Combining evidence for evolutionary constraint and conserved expression data, we previously identified candidate lncRNAs that might play important and conserved roles in brain function. Here, we demonstrate that the sequence and neuronal transcription of lncRNAs transcribed from the previously uncharacterized Visc locus are conserved across diverse mammals. Consequently, one of these lncRNAs, Visc-2, was selected for targeted deletion in the mouse, and knockout animals were subjected to an extremely detailed anatomical and behavioral characterization. Despite a neurodevelopmental expression pattern of Visc-2 that is highly localized to the cortex and sites of neurogenesis, anomalies in neither cytoarchitecture nor neuroproliferation were identified in knockout mice. In addition, no abnormal motor, sensory, anxiety, or cognitive behavioral phenotypes were observed. These results are important because they contribute to a growing body of evidence that lncRNA loci contribute on average far less to brain and biological functions than protein-coding loci. A high-throughput knockout program focussing on lncRNAs, similar to that currently underway for protein-coding genes, will be required to establish the distribution of their organismal functions.

Peer Review status:Peer ReviewedPublication status:PublishedVersion:Full version available to download Funder: Medical Research Council   Funder: Biotechnology and Biological Sciences Research Council   Funder: European Research Council   Notes:This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Bibliographic Details

Journal: Cerebral cortex (New York, N.Y. : 1991)see more from them

Issue Date: 2014-9-10Identifiers

Urn: uuid:85edb607-0e46-45f1-a4af-13aa04fac8ae

Source identifier: 484078

Eissn: 1460-2199

Doi: https://doi.org/10.1093/cercor/bhu196

Issn: 1047-3211 Item Description

Type: Journal article;

Language: eng

Version: Full version available to downloadKeywords: evolutionary conservation knockout mouse noncoding RNA olfactory bulb subventricular zone Tiny URL: pubs:484078

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Autor: Oliver, PL - institutionUniversity of Oxford Oxford, MSD, Physiology Anatomy and Genetics - - - Chodroff, RA - institutionUnivers

Fuente: https://ora.ox.ac.uk/objects/uuid:85edb607-0e46-45f1-a4af-13aa04fac8ae



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