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Reference: Carr, CA, Stuckey, DJ, Tan, JJ et al., (2011). Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study. PloS one, 6 (10), Article: e25669.Citable link to this page:

 

Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study.

Abstract: AIMS: Endogenous cardiac progenitor cells, expanded from explants via cardiosphere formation, present a promising cell source to prevent heart failure following myocardial infarction. Here we used cine-magnetic resonance imaging (MRI) to track administered cardiosphere-derived cells (CDCs) and to measure changes in cardiac function over four months in the infarcted rat heart. METHODS AND RESULTS: CDCs, cultured from neonatal rat heart, comprised a heterogeneous population including cells expressing the mesenchymal markers CD90 and CD105, the stem cell marker c-kit and the pluripotency markers Sox2, Oct3/4 and Klf-4. CDCs (2 × 10(6)) expressing green fluorescent protein (GFP+) were labelled with fluorescent micron-sized particles of iron oxide (MPIO). Labelled cells were administered to the infarcted rat hearts (n = 7) by intramyocardial injection immediately following reperfusion, then by systemic infusion (4 × 10(6)) 2 days later. A control group (n = 7) was administered cell medium. MR hypointensities caused by the MPIOs were detected at all times and GFP+ cells containing MPIO particles were identified in tissue slices at 16 weeks. At two days after infarction, cardiac function was similar between groups. By 6 weeks, ejection fractions in control hearts had significantly decreased (47 ± 2%), but this was not evident in CDC-treated hearts (56 ± 3%). The significantly higher ejection fractions in the CDC-treated group were maintained for a further 10 weeks. In addition, CDC-treated rat hearts had significantly increased capillary density in the peri-infarct region and lower infarct sizes. MPIO-labelled cells also expressed cardiac troponin I, von Willebrand factor and smooth muscle actin, suggesting their differentiation along the cardiomyocyte lineage and the formation of new blood vessels. CONCLUSIONS: CDCs were retained in the infarcted rat heart for 16 weeks and improved cardiac function.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: British Heart Foundation   Funder: Malaysian Ministry of Higher Education   Funder: Marie Curie International   Notes:Copyright 2011 Carr et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website: http://www.plos.org

Journal: PloS onesee more from them

Publication Website: http://www.plosone.org

Issue Date: 2011

pages:Article: e25669Identifiers

Urn: uuid:bac5e5f0-f8c5-4569-9ef8-53ada3f93818

Source identifier: 198760

Eissn: 1932-6203

Doi: https://doi.org/10.1371/journal.pone.0025669

Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Animals Animals, Newborn Rats Myoblasts, Cardiac Myocardial Infarction Ferric Compounds Heart Function Tests Green Fluorescent Proteins Magnetic Resonance Imaging, Cine Treatment Outcome Stem Cell Transplantation Cell Differentiation Time Factors Tiny URL: pubs:198760

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Autor: Carr, CA - institutionUniversity of Oxford Oxford, MSD, Physiology Anatomy and Genetics - - - Stuckey, DJ - institutionUniversity

Fuente: https://ora.ox.ac.uk/objects/uuid:bac5e5f0-f8c5-4569-9ef8-53ada3f93818



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