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Reference: Possingham, John V., (1956). Some effects of minor nutrients on the growth and metabolism of plants. DPhil. University of Oxford.Citable link to this page:


Some effects of minor nutrients on the growth and metabolism of plants

Abstract: Investigations are described which were carried out to analyse the way in which certain mineral element deficiencies restrict the growth and development of plants. The plant system used in this work was excised pea roots grown in sterile culture media, and the deficiencies studied were those of iron, magnesium and molybdenum.Growth was measured at the cell level and related to other characteristics of the system; two different experimental designs being employed to assess the effects of deficiencies. In the first, roots were grown in full nutrient and in deficient media and growth was measured on samples taken after growing periods of 0, 3, 5, 7, 9 and 11 days; while in the second, roots were grown for 7 days in full nutrient and in deficient media and serial one centimetre sections taken from these roots were compared. The first approach assessed the effects of the deficiency on overall growth, and the second gave an indication of the effects of the deficiency on the longitudinal differentiation of pea roots.Both experimental approaches were employed when examining iron and magnesium deficient roots, but only the second when examining molybdenum deficient roots. Roots deficient in iron and magnesium were obtained by culturing tips cut from germinated seeds in deficient media, but two successive tip passages were necessary to obtain roots deficient in molybdenum.Growth was assessed basically in terms of length, cell volume, protein nitrogen, and rate of oxygen uptake. However with iron deficient roots measurements of invertase activity, sensitivity of the oxygen uptake to cyanide, and the frequencies of cells in the different stages of division were also made. The techniques involved in the culture of deficient and full nutrient roots, and the analytical techniques are described.It has been shown that iron deficiency markedly affects the growth and development of excised pea roots. Growth in terms of length and cell number per root is stopped after 7 days and no further increases occur between days 7 and 11. Although iron deficiency stops cell division, measurements made at day 7 indicate that this deficiency does not restrict the process of cell expansion. In fact 7 day old iron deficient roots carry larger cells in the terminal centimetre than full nutrient roots. By 11 days the iron deficient roots have a pronounced swelling at the terminal end, and it is suggested that this is brought about by an abnormal expansion of the cells in the lateral direction.Some cells containing mitotic figures are present in the tips of 7 and 11 day old iron deficient roots. However there are fewer cells in the division stages of prophase and metaphase and practically no cells in the stages of telophase and anaphase in the deficient roots when comparisons were made with full nutrient roots.The protein nitrogen content of iron deficient roots is lower than that of full nutrient roots at day 7, but there is a considerable increase in both deficient and full nutrient roots between days 7 and 11. The trend of the derived quantity, average protein nitrogen content per cell, is the same in both groups of roots up to day 7, but from day 7 to day 11 it increases sharply in the deficient roots but does not change in the full nutrient roots. This result indicates that cell division was not stopped in the deficient roots by a shortage of protein nitrogen as such.At the day 7 stage the distribution of protein nitrogen along the length of deficient roots is different to that in full nutrient roots. The front sections of the deficient roots contain an increased content and the back sections a decreased content when compared with full nutrient roots. On a per cell basis the situation is the same, as the cells in the front sections of deficient roots have a higher average protein content and those in the back sections a lower content when compared with the cells of full nutrient roots.The accumulation of protein nitrogen in the front sections of iron deficient roots is most probably associated with the cessation of active cell division in the meristem. Evidence is available which suggests that under normal conditions the formation and development of cells in the apex of the root is dependent on substrates synthesised in the mature regions of the root and translocated forward. It is considered that in iron deficient roots precursors of protein are no longer removed by the demands of the meristem and they condense to form protein in the regions adjacent to the apex.

Autor: Possingham, John V. - institutionUniversity of Oxford facultyDepartment of Plant Sciences - - - - Bibliographic Details Issue Dat



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