Cutaneous squamous cell carcinoma SCC and the DNA damage response: pATM expression patterns in pre-malignant and malignant keratinocyte skin lesions.Reportar como inadecuado




Cutaneous squamous cell carcinoma SCC and the DNA damage response: pATM expression patterns in pre-malignant and malignant keratinocyte skin lesions. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Reference: Ismail, F, Ikram, M, Purdie, K et al., (2011). Cutaneous squamous cell carcinoma (SCC) and the DNA damage response: pATM expression patterns in pre-malignant and malignant keratinocyte skin lesions. PloS one, 6 (7), Article: e21271.Citable link to this page:

 

Cutaneous squamous cell carcinoma (SCC) and the DNA damage response: pATM expression patterns in pre-malignant and malignant keratinocyte skin lesions.

Abstract: Recent evidence suggests that an initial barrier to the emergence of tumours is a DNA damage response that evokes a counter-response which arrests the growth of, or eliminates, damaged cells. Early precursor lesions express markers of an activated DNA damage response in several types of tumour, with a diminishing response in more advanced cancers. An important marker of DNA damage is ATM which becomes phosphorylated (pATM) upon activation. We have investigated pATM expression patterns in cultured keratinocytes, skin explants and a spectrum of pre-malignant to malignant keratinocyte skin lesions by immunohistochemistry. We found that pATM was mainly localised to the Golgi apparatus, which contrasts with its nuclear localisation in other tissues. Upon UV irradiation there is transient formation of pATM in nuclear foci, consistent with recruitment to the sites of DNA damage. By immunohistochemistry we show pATM expression in precancerous keratinocyte lesions is greater and predominantly nuclear when compared to the invasive lesions where pATM is weaker and predominantly cytoplasmic. Our results are consistent with the hypothesis that the DNA damage response acts as a barrier to cutaneous tumour formation, but also suggests that ATM expression in skin is different compared to other tissues. This may be a consequence of the constant exposure of skin to UVR, and has implications for skin carcinogenesis.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Medical Research Council   Notes:Copyright 2011 Ismail et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website: http://www.plos.org

Journal: PloS onesee more from them

Publication Website: http://www.plosone.org

Issue Date: 2011

pages:Article: e21271Identifiers

Urn: uuid:cba810d2-9c9d-436c-92f7-a25e71b9cc25

Source identifier: 175441

Eissn: 1932-6203

Doi: https://doi.org/10.1371/journal.pone.0021271

Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Humans Golgi Apparatus Cell Nucleus Carcinoma in Situ Carcinoma, Squamous Cell Disease Progression DNA-Binding Proteins Phosphoproteins Tumor Suppressor Proteins Cell Cycle Proteins Ultraviolet Rays Gene Expression Regulation, Neoplastic Cell Line, Tumor Keratinocytes Neoplasm Invasiveness Protein-Serine-Threonine Kinases Keratosis, Actinic Time Factors DNA Damage Ataxia Telangiectasia Mutated Proteins Skin Neoplasms Tiny URL: pubs:175441

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Autor: Ismail, F - - - Ikram, M - - - Purdie, K - - - Harwood, C - - - Leigh, I - - - Storey, A - institutionUniversity of Oxford Oxford

Fuente: https://ora.ox.ac.uk/objects/uuid:cba810d2-9c9d-436c-92f7-a25e71b9cc25



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