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Reference: Kulick, S, Moccia, C, Didelot, X et al., (2008). Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori. PloS one, 3 (11), Article: e3797.Citable link to this page:

 

Mosaic DNA imports with interspersions of recipient sequence after natural transformation of Helicobacter pylori.

Abstract: Helicobacter pylori colonizes the gastric mucosa of half of the human population, causing gastritis, ulcers, and cancer. H. pylori is naturally competent for transformation by exogenous DNA, and recombination during mixed infections of one stomach with multiple H. pylori strains generates extensive allelic diversity. We developed an in vitro transformation protocol to study genomic imports after natural transformation of H. pylori. The mean length of imported fragments was dependent on the combination of donor and recipient strain and varied between 1294 bp and 3853 bp. In about 10% of recombinant clones, the imported fragments of donor DNA were interrupted by short interspersed sequences of the recipient (ISR) with a mean length of 82 bp. 18 candidate genes were inactivated in order to identify genes involved in the control of import length and generation of ISR. Inactivation of the antimutator glycosylase MutY increased the length of imports, but did not have a significant effect on ISR frequency. Overexpression of mutY strongly increased the frequency of ISR, indicating that MutY, while not indispensable for ISR formation, is part of at least one ISR-generating pathway. The formation of ISR in H. pylori increases allelic diversity, and contributes to the uniquely low linkage disequilibrium characteristic of this pathogen.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Sixth Research Framework Programme of the European Union   Funder: Research Training Group   Funder: Deutsche Forschungsgemeinschaft   Funder: German Academic Exchange Service   Funder: Wilhelm Hirte Foundation   Notes:Copyright 2008 Kulick et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website: http://www.plos.org

Journal: PloS onesee more from them

Publication Website: http://www.plosone.org

Issue Date: 2008

pages:Article: e3797Identifiers

Urn: uuid:d5dea737-d5c7-4488-9c3d-0e386d543cfa

Source identifier: 97730

Eissn: 1932-6203

Doi: https://doi.org/10.1371/journal.pone.0003797

Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Humans Helicobacter pylori DNA Glycosylases DNA, Viral Linkage Disequilibrium Recombination, Genetic Mosaicism Genetic Variation Tiny URL: pubs:97730

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Autor: Kulick, S - - - Moccia, C - - - Didelot, X - institutionUniversity of Oxford Oxford, MPLS, Statistics - - - Falush, D - - - Kraft

Fuente: https://ora.ox.ac.uk/objects/uuid:d5dea737-d5c7-4488-9c3d-0e386d543cfa



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