Ab initio identification of novel regulatory elements in the genome of Trypanosoma brucei by Bayesian inference on sequence segmentation.Reportar como inadecuado




Ab initio identification of novel regulatory elements in the genome of Trypanosoma brucei by Bayesian inference on sequence segmentation. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Reference: Kelly, S, Wickstead, B, Maini, PK et al., (2011). Ab initio identification of novel regulatory elements in the genome of Trypanosoma brucei by Bayesian inference on sequence segmentation. PloS one, 6 (10), e25666.Citable link to this page:

 

Ab initio identification of novel regulatory elements in the genome of Trypanosoma brucei by Bayesian inference on sequence segmentation.

Abstract: BACKGROUND: The rapid increase in the availability of genome information has created considerable demand for both comparative and ab initio predictive bioinformatic analyses. The biology laid bare in the genomes of many organisms is often novel, presenting new challenges for bioinformatic interrogation. A paradigm for this is the collected genomes of the kinetoplastid parasites, a group which includes Trypanosoma brucei the causative agent of human African trypanosomiasis. These genomes, though outwardly simple in organisation and gene content, have historically challenged many theories for gene expression regulation in eukaryotes. METHODOLOGY/PRINCIPLE FINDINGS: Here we utilise a Bayesian approach to identify local changes in nucleotide composition in the genome of T. brucei. We show that there are several elements which are found at the starts and ends of multicopy gene arrays and that there are compositional elements that are common to all intergenic regions. We also show that there is a composition-inversion element that occurs at the position of the trans-splice site. CONCLUSIONS/SIGNIFICANCE: The nature of the elements discovered reinforces the hypothesis that context dependant RNA secondary structure has an important influence on gene expression regulation in Trypanosoma brucei.

Publication status:Published Funder: Biotechnology and Biological Sciences Research Council   Funder: Wellcome Trust   Funder: Royal Society   Notes:Copyright 2011 Kelly et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website: http://www.plos.org

Journal: PloS onesee more from them

Publication Website: http://www.plosone.org

Issue Date: 2011

pages:Article: e25666

pages:e25666Identifiers

Urn: uuid:f7fc8445-ced9-4b3b-a84e-ae30b450322d

Source identifier: 186500

Eissn: 1932-6203

Doi: https://doi.org/10.1371/journal.pone.0025666

Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng Keywords: Animals Humans Trypanosoma brucei brucei DNA, Intergenic RNA Splice Sites Nucleotides Oligonucleotide Array Sequence Analysis Sequence Analysis, DNA Base Sequence Regulatory Sequences, Nucleic Acid Genome, Protozoan Polyadenylation Trans-Splicing Computer Simulation Base Composition Bayes Theorem Chromosomes Computational Biology Tiny URL: pubs:186500

Relationships





Autor: Kelly, S - institutionUniversity of Oxford Oxford, MPLS, Plant Sciences - - - Wickstead, B - institutionUniversity of Oxford - -

Fuente: https://ora.ox.ac.uk/objects/uuid:f7fc8445-ced9-4b3b-a84e-ae30b450322d



DESCARGAR PDF




Documentos relacionados