Three-dimensional perfused tumour spheroid model for anti-cancer drug screeningReport as inadecuate




Three-dimensional perfused tumour spheroid model for anti-cancer drug screening - Download this document for free, or read online. Document in PDF available to download.

Reference: Wan, X, Li, Z, Ye, H et al., (2016). Three-dimensional perfused tumour spheroid model for anti-cancer drug screening. Biotechnology Letters.Citable link to this page:

 

Three-dimensional perfused tumour spheroid model for anti-cancer drug screening

Abstract: ObjectiveTo build up an in vitro perfused three-dimensional (3D) spheroid model based on the TissueFlex® system for anti-cancer drug efficacy testing, to closer mimic avascular micro-tissues with inherent oxygen, nutrient and metabolite gradients, and to provide more accurate prediction of drug toxicity and efficacy than traditional in vitro tumour models in conventional static culture well plates.ResultsThe perfused cancer spheroid model showed higher cell viability and increased diameter of spheroid over a relatively long culture period (17 days). Three anti-cancer drugs with different cytotoxic mechanisms were tested. In perfusion, lower cytotoxicity was observed for traditional cytotoxic drug 5-fluorouracil (5-FU), and microtubule-interfering Paclitaxel showed more spheroid integrity interruption. For the hypoxic-dependent drug, tirapazamine (TPZ), there was no significant difference observed between static and perfusion cultures.ConclusionThe perfused cancer spheroid model showed higher cell viability and increased diameter of spheroid over a relatively long culture period (17 days). Three anti-cancer drugs with different cytotoxic mechanisms were tested. In perfusion, lower cytotoxicity was observed for traditional cytotoxic drug 5-fluorouracil (5-FU), and microtubule-interfering Paclitaxel showed more spheroid integrity interruption. For the hypoxic-dependent drug, tirapazamine (TPZ), there was no significant difference observed between static and perfusion cultures.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Biotechnology and Biological Science Research Council   Notes:Copyright © The Author(s) 2016. This article is distributed under the terms of theCreative Commons Attribution 4.0 International License(http://creativecommons.org/licenses/by/4.0/), which permitsunrestricted use, distribution, and reproduction in any medium,provided you give appropriate credit to the original author(s)and the source, provide a link to the Creative Commons license,and indicate if changes were made

Bibliographic Details

Publisher: Springer Netherlands

Publisher Website: http://www.springer.com/

Journal: Biotechnology Letterssee more from them

Publication Website: http://link.springer.com/journal/10529

Issue Date: 2016Identifiers

Urn: uuid:f747f830-ad52-4d0c-9921-ce9ada246c64

Source identifier: 619870

Eissn: 1573-6776

Doi: https://doi.org/10.1007/s10529-016-2035-1

Issn: 0141-5492 Item Description

Type: Journal article;

Language: English

Version: Publisher's versionKeywords: cancer spheroids drug screening perfusion three-dimensional cancer model Tiny URL: pubs:619870

Relationships





Author: Wan, X - institutionUniversity of Oxford Oxford, MSD, Oncology, CRUK-MRC Ox Inst for Radiation Oncology - - - Li, Z - institution

Source: https://ora.ox.ac.uk/objects/uuid:f747f830-ad52-4d0c-9921-ce9ada246c64



DOWNLOAD PDF




Related documents