Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick.Report as inadecuate




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Reference: Patthey, C, Clifford, H, Haerty, W et al., (2016). Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick. Neural development, 11 (1), 3.Citable link to this page:

 

Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick.

Abstract: BackgroundThe cranial sensory ganglia represent populations of neurons with distinct functions, or sensory modalities. The production of individual ganglia from distinct neurogenic placodes with different developmental pathways provides a powerful model to investigate the acquisition of specific sensory modalities. To date there is a limited range of gene markers available to examine the molecular pathways underlying this process.ResultsTranscriptional profiles were generated for populations of differentiated neurons purified from distinct cranial sensory ganglia using microdissection in embryonic chicken followed by FAC-sorting and RNAseq. Whole transcriptome analysis confirmed the division into somato- versus viscerosensory neurons, with additional evidence for subdivision of the somatic class into general and special somatosensory neurons. Cross-comparison of distinct ganglia transcriptomes identified a total of 134 markers, 113 of which are novel, which can be used to distinguish trigeminal, vestibulo-acoustic and epibranchial neuronal populations. In situ hybridisation analysis provided validation for 20/26 tested markers, and showed related expression in the target region of the hindbrain in many cases.ResultsOne hundred thirty-four high-confidence markers have been identified for placode-derived cranial sensory ganglia which can now be used to address the acquisition of specific cranial sensory modalities.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: The Royal Society   Funder: European Molecular Biology Organization   Funder: Elizabeth Hannah Jenkinson Fund   Funder: Fell Fund   Funder: Swedish Society for Medical Research   Funder: Medical Research Council   Funder: Wellcome Trust   Notes:© 2016 Patthey et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Bibliographic Details

Publisher: BioMed Central

Publisher Website: http://www.biomedcentral.com/

Journal: Neural developmentsee more from them

Publication Website: http://neuraldevelopment.biomedcentral.com/

Issue Date: 2016-01

Article Number:ARTN 3

pages:3Identifiers

Urn: uuid:f833f7fd-f3b1-45bf-90b2-f1e3a120c8b9

Source identifier: 601369

Eissn: 1749-8104

Doi: https://doi.org/10.1186/s13064-016-0057-y

Issn: 1749-8104 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Cranial sensory ganglia Viscerosensory neuron Somatosensory neuron Cell type markers Chicken FACS Expression profiling Tiny URL: pubs:601369

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Author: Patthey, C - - - Clifford, H - institutionUniversity of Oxford Oxford, MSD, Physiology Anatomy and Genetics - - - Haerty, W - - -

Source: https://ora.ox.ac.uk/objects/uuid:f833f7fd-f3b1-45bf-90b2-f1e3a120c8b9



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