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Reference: Pawelczyk, S, Scott, KA, Hamer, R et al., (2012). Predicting inter-species cross-talk in two-component signalling systems. PloS one, 7 (5), e37737.Citable link to this page:


Predicting inter-species cross-talk in two-component signalling systems.

Abstract: Phosphosignalling pathways are an attractive option for the synthetic biologist looking for a wide repertoire of modular components from which to build. We demonstrate that two-component systems can be used in synthetic biology. However, their potential is limited by the fact that host cells contain many of their own phosphosignalling pathways and these may interact with, and cross-talk to, the introduced synthetic components. In this paper we also demonstrate a simple bioinformatic tool that can help predict whether interspecies cross-talk between introduced and native two-component signalling pathways will occur and show both in vitro and in vivo that the predicted interactions do take place. The ability to predict potential cross-talk prior to designing and constructing novel pathways or choosing a host organism is essential for the promise that phosphosignalling components hold for synthetic biology to be realised.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Biotechnology and Biological Sciences Research Council   Funder: Engineering and Physical Sciences Research Council   Notes:Copyright 2012 Pawelczyk et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Public Library of Science

Publisher Website:

Journal: PloS onesee more from them

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Issue Date: 2012

pages:Article: e37737


Urn: uuid:f92ccda6-7cfb-4a37-bd3b-3925d74e4c0b

Source identifier: 332915

Eissn: 1932-6203


Issn: 1932-6203 Item Description

Type: Journal article;

Language: eng

Version: Publisher's versionKeywords: Protein Kinases Signal Transduction Phosphorylation Models, Genetic Tiny URL: pubs:332915


Author: Pawelczyk, S - institutionUniversity of Oxford - - - Scott, KA - institutionUniversity of Oxford - - - Hamer, R - institutionUniv



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