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Reference: Patel, Shailendra Bhanubhai., (1984). Characterisation of nuclear sub-structures. Dphil. University of Oxford.Citable link to this page:


Characterisation of nuclear sub-structures

Abstract: When living cells are lysed in non-ionic detergents and 2MNaCl, structures are released that resemble nuclei, termednucleoids. Nucleoids contain tenaciously attached DNA, RNA andprotein. The nature of the interactions of these components ispoorly understood.It is known that the DNA is attached to these structures in alooped configuration, and newly synthesised DNA is found closelyassociated with the attachment sites. Therefore, the speculationthat these attachment sites have a functional signification otherthan for structural purposes has been seriously considered.To investigate these possibilities, the proteins werecharacterised for DNA binding activity, and the presence of anyenzymatic activity. Some of the nucleoid proteins are derived fromthe cell surface, and specific phosphorylating and methylatingactivities were also detected. The significance of these findingsremains to be determined. The study of the DNA-binding activity ishampered by the fact that these proteins are not readilysolubilised away from the nucleic acids. However, DNA-bindingproteins are present in nucleoids. No specificity for DNAsequences was demonstrable, using the protein blotting technique.In the course of these studies, a new technique was devised toenable sequence-binding proteins to be identified.Examination of the DNA close to thl attachment sites shows itto be enriched in transcriptionally active genes, and in a givenpopulation of cells, some genes are closer to the attachment sitesthan others. This supports the idea that genes are specificallyarranged within the nucleus of any cell, and that this position isof functional significance. Direct examination of the most closelyadherent DNA to these structures did not reveal any one DNAsequence that may mediate attachment.

Type of Award:Dphil Level of Award:Doctoral Awarding Institution: University of Oxford Notes:The digital copy of this thesis has been made available thanks to the generosity of Dr Leonard Polonsky


Cook, P. R.More by this contributor



Peter CookMore by this contributor


 Bibliographic Details

Issue Date: 1984Identifiers

Urn: uuid:faf348b8-ddf8-40fa-9b30-03bda8997982

Source identifier: 602831371 Item Description

Type: Thesis;

Language: eng Subjects: Nucleoids Cell nuclei Chromatin Structure DNA-protein interactions RNA-protein interactions Tiny URL: td:602831371


Author: Patel, Shailendra Bhanubhai. - institutionUniversity of Oxford facultyMedical Sciences Division - - - - Contributors Cook, P. R.



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