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Reference: Yang, Z, Haslehurst, P, Scott, S et al., (2016). A compact light-sheet microscope for the study of the mammalian central nervous system. Scientific Reports.Citable link to this page:

 

A compact light-sheet microscope for the study of the mammalian central nervous system

Abstract: Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.

Peer Review status:Peer reviewedPublication status:PublishedVersion:Publisher's version Funder: Engineering and Physical Sciences Research Council   Funder: Biotechnology and Biological Sciences Research Council   Funder: Wellcome Trust   Notes:This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0

Bibliographic Details

Publisher: Nature Publishing Group

Publisher Website: http://www.nature.com/

Journal: Scientific Reportssee more from them

Publication Website: http://www.nature.com/srep/index.html

Volume: 6

Extent: Article: 26317

Issue Date: 2016-04

pages:Article: 26317Identifiers

Urn: uuid:fb0936cf-566b-4d86-9c06-7794b3d0aaec

Source identifier: 615291

Doi: https://doi.org/10.1038/srep26317

Issn: 2045-2322 Item Description

Type: Journal article;

Version: Publisher's version Tiny URL: pubs:615291

Relationships





Author: Yang, Z - - - Haslehurst, P - institutionUniversity of Oxford Oxford, MSD, Pharmacology - - - Scott, S - institutionUniversity of

Source: https://ora.ox.ac.uk/objects/uuid:fb0936cf-566b-4d86-9c06-7794b3d0aaec



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