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Reference: Castello Palomares, A, Fischer, B, Frese, CK et al., (2016). Comprehensive identification of RNA-binding domains in human cells. Molecular Cell.Citable link to this page:


Comprehensive identification of RNA-binding domains in human cells

Abstract: Mammalian cells harbor more than a thousand RNAbinding proteins (RBPs), with half of these employing unknown modes of RNA binding. We developed RBDmap to determine the RNA-binding sites of native RBPs on a proteome-wide scale. We identified 1,174 binding sites within 529 HeLa cell RBPs, discovering numerous RNA-binding domains (RBDs). Catalytic centers or protein-protein interaction domains are in close relationship with RNA-binding sites, invoking possible effector roles of RNA in the control of protein function. Nearly half of the RNA-binding sites map to intrinsically disordered regions, uncovering unstructured domains as prevalent partners in protein-RNA interactions. RNA-binding sites represent hot spots for defined posttranslational modifications such as lysine acetylation and tyrosine phosphorylation, suggesting metabolic and signal-dependent regulation of RBP function. RBDs display a high degree of evolutionary conservation and incidence of Mendelian mutations, suggestive of important functional roles. RBDmap thus yields profound insights into native protein-RNA interactions in living cells.

Publication status:PublishedPeer Review status:Peer reviewedVersion:Publisher's version Funder: European Research Council   Funder: European Molecular Biology Organization   Funder: German Ministry for Science and Education   Notes:© 2016 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (

Bibliographic Details

Publisher: Cell Press

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Journal: Molecular Cellsee more from them

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Issue Date: 2016-07Identifiers


Issn: 1097-4164

Uuid: 6f25d6fc-de12-41e9-acaa-88bc17f96c4f

Urn: uri:6f25d6fc-de12-41e9-acaa-88bc17f96c4f

Pubs-id: pubs:629323 Item Description

Type: journal-article;

Version: Publisher's versionKeywords: RNA RNA binding protein RNA binding domain


Author: Castello Palomares, A - Oxford, MSD, Biochemistry fundingMedical Research Council grantNumberMR-L019434-1 - - - Fischer, B - - -



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