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Reference: Hanke, T, Ondondo, B, Abdul-Jawad, S et al., (2016). Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells. Journal of HIV and AIDS.Citable link to this page:

 

Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells

Abstract: Attachment of the human tissue plasminogen activator signal peptide (tPA-SP) to a variety of proteins has enhanced the induction of both antibody and cellular immune responses in animal models. Here, as part of a continuing effort to improve HIV-1 vaccine immunogenicity, we sought to determine whether or not addition of optimized tPA-SP improves the magnitude and functionality of T cells elicited by vaccines expressing protein HIVconsv derived from highly conserved regions of the HIV-1 proteome. The HIVconsv vaccines were vectored by plasmid DNA, non-replicating simian (chimpanzee) adenovirus ChAdV63 and non-replicating poxvirus MVA. We found that addition of the tPA-SP increased levels of intracellular HIVconsv protein for all the tested vaccines. When administered intramuscularly to the BALB/c mice, a 2- to 3.5-fold increase in frequencies of vaccine-elicited T-cells were observed for the DNA modality, however, there was no consistent significant improvement when using a much more potent heterologous regimen of ChAdV63 prime-MVA boost. Our results concur with previous DNA studies and emphasize that immunopotentiation by the tPA-SP is likely immunogen, vaccine modality, type of immune responses and species specific. Thus, the tPA-SP effect on induction of desired immune responses should be assessed for each vaccine strategy separately.

Publication status:PublishedPeer Review status:Peer reviewedVersion:Accepted manuscriptDate of acceptance:2016-08-29 Funder: Ministry of Higher Education, Kingdom of Saudi Arabia   Funder: International AIDS Vaccine Initiative   Funder: United States Agency for International Development   Notes: © 2016 Ondondo B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Bibliographic Details

Publisher: Sci Forschen

Publisher Website: https://www.sciforschenonline.org/

Journal: Journal of HIV and AIDSsee more from them

Publication Website: https://www.sciforschenonline.org/journals/hiv-aids/index.php

Issue Date: 2016-09-01Identifiers

Uuid: uuid:86b3a2ab-2c7d-403e-98ce-5717d5c97926

Urn: uri:86b3a2ab-2c7d-403e-98ce-5717d5c97926

Pubs-id: pubs:668774 Item Description

Type: journal-article;

Version: Accepted manuscript

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Autor: Hanke, T - Oxford, MSD, NDM, Jenner Institute fundingDepartment for International Development fundingMedical Research Council gra

Fuente: https://ora.ox.ac.uk/objects/uuid:86b3a2ab-2c7d-403e-98ce-5717d5c97926



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