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Reference: Tucker, IM, Petkov, JT, Penfold, J et al., (2016). Adsorption of hydrophobin/β-casein mixtures at the solid-liquid interface. Journal of Colloid and Interface Science, 478, 81-87.Citable link to this page:


Adsorption of hydrophobin/β-casein mixtures at the solid-liquid interface

Abstract: The adsorption behaviour of mixtures of the proteins β-casein and hydrophobin at the hydrophilic solid-liquid surface have been studied by neutron reflectivity. The results of measurements from sequential adsorption and co-adsorption from solution are contrasted. The adsorption properties of protein mixtures are important for a wide range of applications. Because of competing factors the adsorption behaviour of protein mixtures at interfaces is often difficult to predict. This is particularly true for mixtures containing hydrophobin as hydrophobin possesses some unusual surface properties. At β-casein concentrations ⩾0.1wt% β-casein largely displaces a pre-adsorbed layer of hydrophobin at the interface, similar to that observed in hydrophobin-surfactant mixtures. In the composition and concentration range studied here for the co-adsorption of β-casein-hydrophobin mixtures the adsorption is dominated by the β-casein adsorption. The results provide an important insight into how the competitive adsorption in protein mixtures of hydrophobin and β-casein can impact upon the modification of solid surface properties and the potential for a wide range of colloid stabilisation applications.

Publication status:PublishedPeer Review status:Peer reviewedVersion:Accepted manuscriptDate of acceptance:2016-06-01Notes:This is the author accepted manuscript following peer review version of the article. The final version is available online from Elsevier at: 10.1016/j.jcis.2016.06.002

Bibliographic Details

Publisher: Elsevier

Publisher Website:

Journal: Journal of Colloid and Interface Sciencesee more from them

Publication Website:

Volume: 478

Issue Date: 2016-06-02



Eissn: 1095-7103

Issn: 0021-9797

Uuid: uuid:820b258f-5b02-4b7d-95f5-e12fed2dbf36

Urn: uri:820b258f-5b02-4b7d-95f5-e12fed2dbf36

Pubs-id: pubs:628844 Item Description

Type: journal-article;

Language: eng

Version: Accepted manuscriptKeywords: Hydrophilic surface Hydrophobin Mixed proteins Protein adsorption β-Casein Adsorption Caseins Fungal Proteins Particle Size Surface Properties


Autor: Tucker, IM - - - Petkov, JT - - - Penfold, J - Oxford, MPLS, Chemistry, Physical and Theoretical Chem - - - Thomas, R - Oxford, M



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